Identification of novel androgen receptor target genes in prostate cancer

  • Unnati Jariwala1,

    Affiliated with

    • Jennifer Prescott2,

      Affiliated with

      • Li Jia3,

        Affiliated with

        • Artem Barski1,

          Affiliated with

          • Steve Pregizer1,

            Affiliated with

            • Jon P Cogan1,

              Affiliated with

              • Armin Arasheben1,

                Affiliated with

                • Wayne D Tilley5,

                  Affiliated with

                  • Howard I Scher6,

                    Affiliated with

                    • William L Gerald6,

                      Affiliated with

                      • Grant Buchanan2, 3, 5,

                        Affiliated with

                        • Gerhard A Coetzee2, 3 and

                          Affiliated with

                          • Baruch Frenkel1, 4Email author

                            Affiliated with

                            Contributed equally
                            Molecular Cancer20076:39

                            DOI: 10.1186/1476-4598-6-39

                            Received: 13 March 2007

                            Accepted: 06 June 2007

                            Published: 06 June 2007



                            The androgen receptor (AR) plays critical roles in both androgen-dependent and castrate-resistant prostate cancer (PCa). However, little is known about AR target genes that mediate the receptor's roles in disease progression.


                            Using Chromatin Immunoprecipitation (ChIP) Display, we discovered 19 novel loci occupied by the AR in castrate resistant C4-2B PCa cells. Only four of the 19 AR-occupied regions were within 10-kb 5'-flanking regulatory sequences. Three were located up to 4-kb 3' of the nearest gene, eight were intragenic and four were in gene deserts. Whereas the AR occupied the same loci in C4-2B (castrate resistant) and LNCaP (androgen-dependent) PCa cells, differences between the two cell lines were observed in the response of nearby genes to androgens. Among the genes strongly stimulated by DHT in C4-2B cells – D-dopachrome tautomerase (DDT), Protein kinase C delta (PRKCD), Glutathione S- transferase theta 2 (GSTT2), Transient receptor potential cation channel subfamily V member 3 (TRPV3), and Pyrroline-5-carboxylate reductase 1 (PYCR1) – most were less strongly or hardly stimulated in LNCaP cells. Another AR target gene, ornithine aminotransferase (OAT), was AR-stimulated in a ligand-independent manner, since it was repressed by AR siRNA knockdown, but not stimulated by DHT. We also present evidence for in vivo AR-mediated regulation of several genes identified by ChIP Display. For example, PRKCD and PYCR1, which may contribute to PCa cell growth and survival, are expressed in PCa biopsies from primary tumors before and after ablation and in metastatic lesions in a manner consistent with AR-mediated stimulation.


                            AR genomic occupancy is similar between LNCaP and C4-2B cells and is not biased towards 5' gene flanking sequences. The AR transcriptionally regulates less than half the genes nearby AR-occupied regions, usually but not always, in a ligand-dependent manner. Most are stimulated and a few are repressed. In general, response is stronger in C4-2B compared to LNCaP cells. Some of the genes near AR-occupied regions appear to be regulated by the AR in vivo as evidenced by their expression levels in prostate cancer tumors of various stages. Several AR target genes discovered in the present study, for example PRKCD and PYCR1, may open avenues in PCa research and aid the development of new approaches for disease management.



                            androgen receptor


                            chromatin immunoprecipitation


                            ChIP Display


                            prostate cancer


                            polyacrylamide gel electrophoresis




                            prostate specific antigen 1


                            charchoal stripped serum


                            fetal bovine serum


                            androgen responsive element


                            real time quantitative polymerase chain reaction


                            estrogen receptor


                            serial analysis of binding elements


                            sequence tag analysis of genomic enrichment


                            chromatin immunoprecipitation paired-end ditag sequencing


                            genome mapping technique


                            serial analysis of chromatin occupancy


                            tethered Dam methyltransferase identification


                            pyrroline 5-carboxylate


                            D-dopachrome tautomerase


                            cysteine-rich with EGF-like Domains 2


                            protein kinase C delta


                            glutathione S-transferase theta 2


                            transient receptor potential (TRP) subfamily V member 3


                            pyrroline-5-carboxylate reductase 1


                            adaptor-related protein complex 2 alpha 2 subunit


                            Acyl-Coenzyme A binding domain containing 6


                            silent mating type information regulation 2 (sirtuin 7)


                            mof4 family associated protein 1


                            v-maf musculoaponeurotic fibrosarcoma oncogene homolog G


                            ornithine amino transferase


                            carbohydrate kinase like


                            mannosidase 2 alpha B2


                            LIM homeobox 4


                            alpha 1,6 mannosyltransferase


                            centrosomal protein 350 kDa


                            bromodomain adjacent to zinc finger domain 1B




                            kinesin family member 1A


                            phospholysine phosphohistidine inorganic pyrophosphate phosphatase


                            transient receptor potential (TRP) subfamily V member 1


                            williams beuren syndrome chromosome region 27


                            solute carrier family 22 (organic anion transporter)


                            frizzled homolog 9


                            quiescin Q6


                            mitogen-activated protein kinase kinase kinase 7 interacting protein 1




                            mucin 6


                            cholinergic receptor, muscarinic 1


                            williams beuren syndrome chromosome region 28.


                            Prostate Cancer (PCa) is the most commonly diagnosed non-cutaneous cancer and the second leading cause of cancer-related mortality in men [1]. Prostate development and carcinogenesis are highly androgen dependent [2, 3]. By regulating cell proliferation, differentiation and apoptosis the androgen receptor (AR) plays a pivotal role in PCa progression, as well as in normal prostate development [24]. AR-mediated PCa growth is initially hormone-dependent, and men failing surgical and radiation therapy are therefore subjected to androgen ablation therapy [5]. Androgen ablation in these cases almost always leads to tumor regression, but this is inevitably followed by recurrence of PCa due to the development of castrate-resistant and often metastatic disease.

                            Although most recurrent PCa tumors are castrate-resistant, AR expression and function are maintained in advanced disease [6, 7] and the growth of ablation-resistant PCa cells remains AR dependent as exemplified by the following three lines of evidence. Disruption of the AR by a specific antibody or ribozyme inhibited proliferation in ablation-resistant PCa cells in the absence of androgens [8]. Increased AR expression was necessary and sufficient to convert androgen-sensitive PCa to an ablation-resistant state [9]. Finally, specific expression in mouse prostate epithelial cells of an AR transgene containing a gain-of-function mutation (with increased basal activity and response to coregulators), resulted in PCa development in 100% of the animals [10] proving that aberrant AR signaling was sufficient to cause PCa and that under certain conditions the AR acts as an oncogene.

                            As AR is a transcription factor, its oncogenic functions are likely mediated through specific target genes. Prostate specific antigen (PSA), the best studied AR target gene, is thought to contribute to PCa progression through its protease activity [11] and its ability to induce epithelial-mesenchymal transition and cell migration [12]. Other AR target genes implicated in PCa progression are FGF8 [13], Cdk1 and Cdk2 [14], as well as PMEPA1 [15] and TMPRSS2 [16]. Interestingly, the AR response mechanism of TMPRSS2 drives oncogenic Ets family members in many castrate resistant tumors due to TMPRSS2:Ets chromosomal translocations [17, 18]. However, additional, yet unidentified target genes most likely contribute to the tumorigenic activity of the AR in PCa. The present study was undertaken to identify such genes based on their physical interaction with the AR. C4-2B human PCa cells, a model for castrate-resistant disease, were subjected to a procedure called Chromatin Immunoprecipitation (ChIP) Display (CD) [19] and 19 novel regions occupied by the AR were discovered. The expression patterns of genes within the AR-occupied loci, along with functions attributed to these genes, render some of them potential PCa therapeutic targets.


                            ChIP Display of AR targets in C4-2B cells: an example

                            To identify AR targets in PCa, we employed ChIP Display, a newly developed method for the identification of regions occupied by transcription factors in living cells [19]. C4-2B human PCa cells were stimulated by androgens for 4 hours and ChIP was performed with either AR or IgG control antibodies. The purified DNA was digested with Ava II in order to standardize all DNA fragments representing each AR-occupied region to one size. The Ava II fragments were amplified using ligation-mediated PCR with each of 36 possible nested primer combinations [19]. The use of nested primers reduces ChIP noise because all the fragments representing a given locus are amplified with the corresponding primer combination, while non-specifically precipitated fragments are scattered, i.e. amplified with other primer combinations [19]. The PCR products are subsequently resolved by polyacrylamide gel electrophoresis (PAGE). Figure 1 describes an example of the procedure leading to the identification of one novel target, and Table 1 summarizes all the AR targets identified in this study.
                            Table 1

                            AR targets identified in this study

                            CD Primers1


                            Ava II – Ava II3

                            Nearby Genes4

                            Position of CD Hit Relative to gene

                            ChIP validation5




                            AT, TA


                            30,152,547 – 30,152,728

                            Nearest gene is 626-kb away



                            AA, TC


                            178,433,288 – 178,433,456

                            QSCN6[63, 67]

                            exon 13




                            LHX4 [68]

                            32.7-kb 5'






                            90.6-kb 3'


                            AT, AT


                            241,348,804 – 241,348,990


                            intron 23/exon 24





                            62.4-kb 3'


                            TT, TT


                            53,169,093 – 53,169,401

                            PRKCD[51, 53]

                            0.8-kb 5'



                            AT, AC


                            6,644,411 – 6,644,619


                            intron 5





                            48.7-kb 5'


                            AA, TC


                            72,483,118 – 72,483,317


                            2.7-kb 5'





                            10k-b 3'


                            AT, AG


                            72,922,165 – 72,922,474


                            4-kb 3'





                            27.4-kb 5'


                            CLDN4 [70]

                            37.2-kb 3'


                            AA, AG


                            143,094,298 – 143,094,518

                            Nearest gene is 197kb away [64]



                            AC, TC


                            24,584,349 – 24,584,579


                            intron 2



                            AT, AG


                            126,072,189 – 126,072,473


                            3.4-kb 3'





                            67.9-kb 5'


                            AC, TC


                            1,017,234 – 1,017,529


                            10-kb 5'





                            15-kb 3'


                            AT, AT


                            62,532,814 – 62,532,977


                            intron 2





                            23.9-kb 5'



                            87.3-kb 5'


                            AT, AT


                            134,102,859 – 134,103,167

                            Nearest gene is 315-kb away



                            AT, AT


                            86,510,091 – 86,510,360

                            Nearest gene is 959-kb away



                            AT, AC


                            3,446,846 – 3,447,080


                            exon 1/intron 1





                            11.4-kb 3'


                            TRPV3 [60, 61]

                            39-kb 5'


                            AC, TG


                            77,480,155 – 77,480,527


                            1.5-kb 5'




                            PYCR1 [36, 50]

                            2.5-kb 3'


                            SIRT7 [71, 72]

                            12-kb 5'


                            AA, AA


                            22,655,127 – 22,655,462


                            exon 4/intron 4





                            3.1-kb 5'


                            AG, AG


                            38,101,611 – 38,101,989


                            intron 2





                            25-kb 5'


                            AC, TC


                            48,707,684 – 48,707,956


                            1-kb 3'





                            10-kb 5'


                            1CD primers are defined based on two variable nucleotides as described in Methods.

                            2Cytogenetic band containing the CD hit.

                            3Absolute positions of the Ava II sites flanking the fragment displayed by PAGE.

                            4The nearest Refseq gene annotated in Ensembl [65] is shown in bolded and italicized text. Published papers suggesting relevance to cancer are referenced near the respective gene name.

                            5Number of independent conventional ChIP assays in which AR occupancy was confirmed. nd, not determined.

                            Figure 1

                            ChIP Display (CD) demonstrates a putative AR target. A) CD Gel. C4-2B cells were treated with 10 nM DHT for 4 hours to enhance AR association with target loci. Two independent AR ChIPs, and IgG control ChIPs were subjected to the CD procedure as described in Methods. In the example shown here, PCRs were performed with the 'AC' and the 'TG' PCR primers (see Methods and Additional file 1) with the annealing temperature set at either 70°C or 71°C as indicated. Amplified products were resolved using 8% PAGE and visualized by EtBr staining. The arrowheads point at bands amplified more prominently in the AR compared to the Control (IgG) lanes. M, marker DNA; numbers above bands indicate size in bps. B) Re-amplification and digestion. The two bands indicated in panel A by arrowheads were excised, purified and re-amplified with the same 'AC' and 'TG' primers used for CD. The products were subjected to secondary digestion with the indicated enzymes, followed by agarose gel electrophoresis. Arrowheads point at similar Hae III sub-fragments obtained from the two AR ChIPs. – C, no template control, UC, uncut, M, marker DNA. C) Mapping of AR target. The Hae III subfragments from B were excised, purified and sequenced. By blasting against the human genome using Ensembl [65], both sequences mapped to chromosome 17q25.3, ~1.5-kb upstream of the MAFG gene and ~2.5-kb downstream of the PYCR1 gene as shown in the diagram. The two genes are transcribed in the same direction as indicated by the horizontal arrows. pA, polyadenylation signal. The AR binding region discovered through CD (''hit'') abuts a CpG island (bottom, striped rectangle), but does not overlap with any repetitive elements (bottom, black rectangles). Several AREs (checkerboard triangles) were identified in this region using Consite [66]. D) Validation of target by conventional ChIP analysis. AR occupancy at the PYCR1/MAFG locus was tested by conventional ChIP assay. The PSA enhancer serves as positive control. A non-target locus serves as the negative control. Genomic DNA was used to demonstrate that the ChIP amplification was performed within a dynamic range. – C, no template control. M, marker DNA.

                            The example shown in Figure 1A entails the amplification and PAGE of two independent AR-ChIPs and two mock ChIPs using one of the 36 primer combinations – the 'AC' and the 'TG' primer (see Methods and Additional file 1). The arrowheads in Figure 1A point to bands more prominently amplified in the AR ChIPs as compared to the IgG ChIPs. These bands, representing a putative AR binding region, were excised, reamplified and further characterized by secondary restriction digests and agarose gel electrophoresis (Figure 1B). The major Hae III digestion product from each of the two ChIPs was sequenced and mapped to human chromosome 17q25.3, 1.5-kb upstream of the MAFG gene and 2.5-kb 3' of the PYCR1 gene (Figure 1C). The Ava II fragment displayed in the original PAGE (Figure 1A), depicted in Figure 1C as "hit", does not contain repetitive sequences and is located between two canonical Androgen Receptor Elements (AREs) (Figure 1C). A 2.4-kb CpG island is present adjacent to the hit (Figure 1C).

                            To validate AR occupancy at the region described above, we performed conventional ChIP assays with locus-specific primers (see Additional file 1). Four independent experiments with C4-2B cells showed that the PYCR1/MAFG locus was enriched in AR ChIPs as compared to paired IgG control ChIPs (Table 1, and see a representative result in Figure 1D).

                            ChIP Display discloses 19 novel AR binding sites in PCa cells

                            The CD procedure, exemplified above for the 'AC' and 'TG' primer pair, was performed using all 36 possible primer combinations [19], resulting in the identification of 19 novel AR-occupied regions in C4-2B cells (Table 1). AR occupancy at the novel AR binding regions was confirmed in independent conventional ChIP assays of C4-2B cells (Table 1). Whereas only four of the 19 AR binding regions were up to 10-kb 5' of the nearest gene (indicated by bolded and italicized text in Table 1), many of the binding regions were either within the body of annotated genes (8 of the 19 regions) or up to 4-kb 3' of the nearest gene (3 of the 19 regions), indicating that AR-bound regions are not preferentially found within so-called 5'-flanking gene regulatory sequences. Four of the 19 CD hits were mapped to regions more than 197-kb away from any annotated gene (Table 1).

                            An important enigma in prostate cancer research is the molecular nature of the transition from androgen-dependent to castrate-resistant disease. In this context, the C4-2B cell line serves as a model of the latter, whereas its parent cell line, LNCaP, serves as a model of the former [20]. We speculated that many of the AR-occupied regions in C4-2B cells could become targets for this transcription factor only during the transition from androgen dependence to castrate-resistance and would therefore not be occupied by the AR in LNCaP cells. However, results of ChIP analysis in LNCaP cells were inconsistent with this notion, as 16 of 17 regions that we tested, which were occupied by the AR in C4-2B cells, were also occupied in LNCaP cells at least in one conventional ChIP assay (Table 1). Be that as it may, several of the AR-occupied regions are located near genes that have been linked to prostate or other cancers (see Discussion below and references in Table 1).

                            AR occupied regions are associated with DHT-stimulated and DHT-repressed genes in C4-2B cells

                            One of the goals of this study was to identify primary AR-responsive target genes in PCa cells. Of the 19 AR-occupied regions, 15 were within 10-kb of Refseq-annotated genes. We initially measured the androgen responsiveness of genes nearest to each of these 15 AR-occupied regions (gene names bolded and italicized in Table 1). C4-2B cells were depleted from steroids, and treated with DHT or vehicle for 0, 2, 4, 8, 16, 24 or 48 hours. Gene expression was assessed by RT-qPCR. Of the 15 genes nearest AR occupied regions, expression of all but SLC22A8 was detectable, and only 6 of the remaining 14 genes responded to DHT treatment in a consistent manner. CRELD2, PRKCD and GSTT2 were stimulated (Figure 2B, C, D, solid lines), whereas MUC6, KIAA1217, and WBSCR28 were repressed (Figure 2ZC, ZE, ZF, solid lines). Because the remaining 8 of 15 genes nearest the AR occupied regions did not respond to DHT, we tested the expression of 19 additional nearby genes, up to 100-kb away from AR occupied regions. Of these 19 genes, the expression of all but AQP12 was detectable, but only eight responded to DHT treatment. DDT, TRPV3, PYCR1, AP2A2, ACBD6, SIRT7 and MRFAP1 were stimulated (Figure 2A and 2E–J, solid lines), and CHRM1 was repressed (Figure 2ZD). Altogether, of 32 genes within 100-kb from AR-occupied regions, many of which have been implicated in cancer progression (see references next to gene names in Table 1), ten were stimulated and four were repressed in DHT-treated C4-2B cells. More detailed investigation of the repressed genes is described elsewhere [21]. Notably, there were four loci in bands 2q37.3, 7q11.23, 10q26.13 and 22q13.1, where no nearby genes responded to DHT despite AR occupancy (Table 1 and Figure 2).
                            Figure 2

                            Gene expression analysis. C4-2B (solid lines) and LNCaP cells (broken lines) were maintained in 5% CSS-containing medium for three days, and then re-fed (time 0) with the same medium supplemented with either 10 nM DHT or ethanol vehicle. RNA was extracted at the indicated time points during the time course and expression of the specified genes was measured by RT-qPCR. Expression levels relative to 18S rRNA (which itself stayed stable throughout the time course) are shown with the 0 time values defined as 1 for each cell line. Representative data is shown from one of two independent experiments with n = 3, except for panels 2L, O, U, Y and ZC, where the C4-2B data is derived from 6 measurements (see Additional file 3 for the complete set of raw data). Error bars are SEM. Genes are roughly ordered based on the DHT-responsiveness in C4-2B cells, with stimulated genes first (panels A-J) to repressed genes last (panels ZC-ZF). TRVP3 mRNA was barely detectable in LNCaP cells.

                            AR-dependent, DHT-independent regulation of OAT and MRFAP1

                            Genes near AR-occupied regions that did not respond to DHT could still be regulated by the AR in a ligand-independent manner. To address this possibility, we treated C4-2B cells with AR siRNA duplexes [22] and assessed the effects on gene expression in the absence (and presence – as control) of DHT. Of eight genes near the four AR-occupied regions that were not associated with DHT-responsiveness in C4-2B cells, we found one, OAT, which was repressed in three of three siRNA experiments (Figure 3A), suggesting that it is indeed stimulated by the AR in the absence of ligand, despite its DHT non-responsiveness (Figure 2L). The other seven genes, KIF1A, AQP12, FZD9, BAZ1B, LHPP, SYNGR1 and MAP3K7IP1 did not respond to the siRNA treatment (data not shown), suggesting that AR occupancy at these loci may be without functional consequences in cultured C4-2B cells.
                            Figure 3

                            Effects of AR siRNA-knockdown on gene expression. C4-2B cells were treated with AR siRNA (white bars) or a non-specific siRNA (black bars), followed by administration of either DHT (10 nM) or Ethanol vehicle for 16 hours. Expression levels of the indicated genes were analyzed in triplicate by RT-qPCR and corrected for 18S rRNA levels. Values measured with the non-specific siRNA and ethanol were defined as 1. Results (Mean ± SD) are representative of three independent experiments.

                            As controls for the AR siRNA experiments we also measured expression of AR itself and the DHT-stimulated genes PSA [23], PRKCD, PYCR1 and MRFAP1 (Figure 2C, F, J). As expected, the AR knockdown (Figure 3B) was associated with loss of DHT-stimulation (Figure 3C, D, and 3E). Interestingly, however, one of these controls, MRFAP1, displayed an unexpected phenotype. In addition to the DHT-stimulation, it was reproducibly stimulated in cells treated with AR siRNA (Figure 3F). Taken together, our data suggest that unliganded AR supports basal OAT expression (Figure 3A) without further stimulation by DHT (Figure 2L), while basal MRFAP1 expression is suppressed by unliganded AR (Figure 3F), yet stimulated by DHT (Figure 2J).

                            Differential regulation of genes near AR-occupied regions in LNCaP versus C4-2B cells

                            Although most of the regions occupied by the AR in C4-2B cells (a model of castrate-resistant PCa) were also occupied in LNCaP cells (a model of androgen-dependent PCa) (Table 1), we suspected that the functional consequences of AR occupancy at these loci might differ between the two cell lines. We therefore complemented the DHT time course studies in C4-2B cells (Figure 2, solid lines) with parallel expression analysis of the same genes in LNCaP cells under a similar experimental protocol (Figure 2, dashed lines). Both similarities and differences between the two cell lines were observed. The three genes most strongly stimulated by DHT in C4-2B cells – DDT, CRELD2 and PRKCD – were also stimulated in LNCaP cells (Figure 2A, B, C), although the stimulation of DDT and PRKCD was more modest in LNCaP cells. Genes that were more moderately stimulated by DHT in C4-2B cells were slightly (PYCR1, Figure 2F) or not at all stimulated in LNCaP cells (GSTT2, AP2A2, ACBD6, and SIRT7; Figure 2, panels D, G, H, I). Repressed genes displayed a mirror image. The four genes most strongly repressed in C4-2B cells – MUC6, CHRM1, KIAA1217, and WBSCR28 – were also repressed in LNCaP cells, but repression generally occurred faster in the C4-2B cells (Figure 2ZC, ZD, ZE, ZF). Subtle responses to DHT were less consistent between C4-2B and LNCaP cells, although three genes, CARKL, MAN2B2, and LHX4, displayed remarkably similar expression patterns (Figure 2, panels M, N, O).

                            An emerging concept in PCa research is that ligand-independent AR-mediated gene expression contributes to the acquisition of a castrate-resistant growth state. If the derivation of C4-2B from LNCaP cells [20] were associated with such a mechanism, then one could expect expression of some genes near AR-occupied regions to be higher in hormone-deprived C4-2B as compared to hormone-deprived LNCaP cells. We therefore compared expression of the 32 genes near the AR-occupied regions between the two cell lines, and found four that were expressed in C4-2B cells at levels between 2 and 12-fold higher than in LNCaP cells (Figure 4). Not surprisingly, one of these genes was OAT, which was repressed after AR knockdown in C4-2B cells (Figure 3A). The other three were QSCN6, GSTT2, and TRPV3. Interestingly, two genes, KIF1A and MAN2B2, were 2 fold less expressed in C4-2B than in LNCaP cells. Thus, differences in gene expression between LNCaP and C4-2B cells, both under androgen deprivation and after DHT stimulation, may be involved in mechanisms of progression from early to late stage disease.
                            Figure 4

                            Gene expression in C4-2B versus LNCaP cells. RNA was extracted from C4-2B and LNCaP cultures that were maintained for two days in CSS-supplemented medium. Gene expression was analyzed side-by-side by RT-qPCR and corrected for 18S rRNA. Bars represent the comparative ratio between the expression in C4-2B and LNCaP cells, where the expression level in LNCaP cells is defined as 1. Included in this Figure are only genes for which the expression levels were significantly different between the two cell lines in two independent experiments (n = 3; Mean ± SD). TRPV3 mRNA was detected in LNCaP cells in only one of the three measurements, and this value was used as the upper limit for TRPV3 expression in these cells. See additional file 4 for details.

                            Clinical relevance of novel AR target genes

                            To examine whether genes found in proximity to AR-occupied regions in our culture model are potentially regulated by the AR during PCa progression, we mined our microarray database of gene expression profiles in PCa tumors [24]. Figure 5 illustrates expression of the in vitro CD-disclosed genes in 23 untreated primary PCa tumors, 17 primary tumors after 3 months of androgen ablation therapy and 7 AR-positive metastatic tumors. Expression of several of these genes was consistent with in vivo regulation by the AR. As shown in Figure 5, Group II, the mRNAs for PYCR1, DDT, PRKCD, and CRELD2, which were DHT-stimulated in vitro (Figure 2), were decreased in the androgen-ablated as compared to the primary untreated tumors. Furthermore, when compared to the androgen-ablated tumors, the expression of these four genes was elevated in the metastatic tumors (Figure 5), presumably due to reactivation of the AR [5, 25]. The similarity between the expression profiles of the CD-disclosed targets PYCR1, DDT, PRKCD, and CRELD2 in the clinical samples and those of the established AR target genes PSA/KLK3 [11] and TMPRSS2 [16] (Figure 5, Group I) suggests that the four genes discovered in our in vitro study are indeed AR targets in vivo. Interestingly, expression of ALG12 and CHRM1, which were unresponsive or even repressed by DHT in vitro, were decreased in androgen-ablated as compared to untreated primary tumors (Figure 5, group II), suggesting positive regulation by the AR in vivo, possibly via mechanisms not operative in our cell culture system. Five probesets displayed a profile indicative of AR-mediated repression in vivo (Figure 5, Group III). Of the corresponding five genes, KIAA1217 was strongly inhibited, while QSCN6 and SYNGR1 were only slightly inhibited by DHT in vitro (Figure 2Z and 2ZB). Notably, the evidence for KIAA1217 repression in vivo was provided by only one probeset (located at the 3'UTR, close to the region targeted by our RT-qPCR primers) and not by five other KIAA1217 probesets present on the array (Figure 5, Group IV). The remaining two genes in Group III (Figure 5), MRFAP1 and OAT, appear to be negatively regulated by the AR in vivo, although they were stimulated or non-responsive to DHT in vitro (Figures 2J and 2L). Interestingly, both these genes were regulated by unliganded AR in vitro (Figure 3). Thus, the expression profiles in the PCa biopsies suggest AR-mediated regulation of CD-disclosed AR target genes in vivo, although the nature of the in vivo response is not always consistent with that seen in vitro.
                            Figure 5

                            Expression of CD-disclosed genes in PCa tumors. RNA from 47 PCa tumors (columns) was analyzed using Affymetrix U95 A-E microarray sets (21) and results are mined for all probesets (rows) interrogating each of the 32 CD-disclosed genes (Table 1). Heat map shows relative expression for each of the indicated probesets, where darker shades represent higher mRNA levels. Tumors included 23 primary prostate cancers from patients not receiving therapy (primary), 17 primary prostate cancers following 3-month neoadjuvant androgen ablation therapy (primary+AAT), and 7 AR-positive metastatic lesions (mets). All Grade A probesets interrogating each gene are shown, except for probesets 59776_at (WBSCR28) and 36904_at (KIF1A), which did not detect significant expression in any sample. Samples are grouped and ranked as follows. Group I – probesets for the known AR-stimulated genes KLK3/PSA and TMPRSS2. Group II – probesets exhibiting statistically greater mean expression in untreated compared to AAT-treated primary PCa samples (p < 0.05), thereby representing putative AR-stimulated genes. Group III – probesets exhibiting statistically lower mean expression in untreated compared to AAT-treated PCa samples (p < 0.05), thereby representing putative AR-repressed genes. Group IV – probesets exhibiting no statistical difference between samples without or with AAT. Probesets in Groups II-IV are ranked by p-value in descending order.


                            AR occupancy is not biased towards 5' promoter-proximal regions

                            The classical view of gene regulation places 5'-flanking sequences at the center stage. Consistent with this view, functional AREs have been mapped within 0.5-kb upstream of the AR-responsive genes probasin, KLK2 and KLK3 (PSA) [2628]. While four of the 19 AR-occupied regions disclosed in our study were located within 10-kb upstream of annotated transcription start sites, many more were found within gene bodies (8/19) or within the 4-kb sequences downstream from the 3' ends of annotated genes. Our findings are consistent with several recent genome-wide location analyses of other transcription factors. For example, only 4% of estrogen receptor (ER) binding sites were mapped to 1-kb promoter-proximal regions by ChIP-chip analysis [29]. Similarly, genome-wide location analysis indicates that p53 has no preference for binding to 5' promoter-proximal regions [30]. Thus, accumulating evidence suggest that promoter-proximal regions constitute only a small fraction of mammalian gene regulatory sequences.

                            Many of the AR-occupied regions identified in the present study, which cannot be designated classical 5' promoter regions, were still close to annotated genes that they could potentially regulate. There is no consensus as to how far a transcription factor-binding region should be in order to be considered a putative cis-acting regulatory domain for a given gene. Values 1-kb, and up to 100-kb from the transcription start site have been used by various investigators [29, 30], but experimental evidence in support of any value is scarce. Systematic analyses of transcription factor binding regions across the genome have become feasible only recently. Such studies, including the present one, illustrate the need for mutagenesis of transcription factors binding regions that are distant from annotated genes in order to identify functionally relevant regions. In particular, it would be interesting to decipher the role of binding regions located hundreds of kbs away from the nearest annotated gene. Such regions may still regulate distant annotated genes on the same [31] or even other chromosomes [32], or they may regulate nearby unannotated transcripts [33].

                            AR location analysis discloses ligand-independent, AR-dependent gene regulation

                            Comprehensive gene expression analysis is frequently employed for the discovery of target genes for transcription factors, including the AR [24, 3436]. Such expression analyses cannot differentiate between direct and indirect targets and they do not provide information on the location of regulatory elements. Another, frequently under-appreciated limitation of expression studies is that they only disclose target genes that respond to the transcription factor of interest under the specific experimental conditions utilized by the investigator. In contrast, ChIP Display and other approaches for location analysis (see below) rely on physical interaction, not gene expression. These approaches allow the discovery of target genes that expression studies would potentially miss. For example, in the present study, we discovered OAT as an AR-regulated gene, although it did not respond to DHT. AR-target genes could also be missed in expression-based studies because of the limited sensitivity and specificity of microarray hybridization as compared to RT-qPCR. Experimental approaches for location analysis are constantly improving and include many that are far more comprehensive than ChIP Display, for example ChIP-chip [3739], SABE [40], STAGE [41], ChIP-PET [30], GMAT [42], SACO [43] and DamID [44]. However, the present study demonstrates that important information can be obtained with ChIP Display, a relatively inexpensive sampling method that can be performed in any molecular biology laboratory. Of course, each of the expression and the location approaches for target identification should ideally be complemented appropriately. In the present study, we showed that many (but not all) of the genes near AR-occupied regions are DHT-responsive. For OAT, which was disclosed here by ChIP Display and could not have been disclosed by comprehensive analysis of gene expression in response to androgen treatment, we used siRNA knockdown to demonstrate the ligand-independent regulation by the AR. Furthermore, of the genes near AR-occupied regions that responded to neither DHT nor AR siRNA in our study, some may be AR-regulated under specific, possibly transient physiological or pathological conditions not modeled by the experimental systems we employed. This is particularly important in the context of castrate-resistant PCa, where AR activation can occur through various signaling pathways, including Her2, AKT, and MAPK [25].

                            Differential basal gene expression in C4-2B versus LNCaP cells

                            The AR plays critical roles during all stages of PCa progression [5, 9, 45]. It is not clear, however, whether AR regulates different sets of genes before and after ablation therapy. In our study, AR occupancy at most of the regions disclosed by CD was similar in LNCaP and C4-2B cells, models of early and late stage PCa, respectively. Our data is therefore consistent with the idea that the AR continues to regulate the same genes before and after ablation therapy, but that the nature of this regulation alters during disease progression. For many genes near AR-occupied regions, ligand-bound AR had the same qualitative effects in the two cell lines, except they were stronger in the C4-2B as compared to the LNCaP model (e.g., DDT, PRKCD, GSTT2, PYCR1; Figure 2). Some other genes near AR-occupied regions were found to express at higher basal levels in C4-2B as compared to LNCaP cells (e.g., OAT, GSTT2, TRPV3; Figure 4). The higher basal expression of these genes could be a direct result of ligand-independent activation by the AR due to, for example, cofactor expression [46, 47] and/or chromatin reorganization [22]. Accumulation of mutations during the derivation of C4-2B from LNCaP cells could also contribute to differential basal and DHT-responsive expression, although the two cell lines are mostly isogenic as indicated by our microsatellite analysis (see Additional file 2).

                            Evidence from PCa biopsies for in vivo AR-mediated regulation of genes disclosed by ChIP Display

                            In the present study, we included analysis of the ChIP Display-disclosed genes for their expression in PCa biopsies. This analysis provided evidence that AR regulates in vivo several of the ChIP Display-disclosed genes. This notion, however, remains tentative because the clinical material used for the microarray expression analysis is no longer available for confirmation by RT-PCR. To increase our confidence in the microarray data, we only considered results from probesets that are considered highly reliable (Affymetrix' grade A annotation). Results from the PCa biopsies were consistent with those from the in vitro gene expression analysis for the positively regulated genes PYCR1, DDT, PRKCD, and CRELD2. However, ALG12 and TRPV1, which appear to be stimulated by the AR in vivo, were not responsive to either DHT or AR siRNA in vitro. The in vivo and in vitro analyses were less well correlated for negatively regulated genes. Only one of six probesets interrogating KIAA1217 expression indicated repression in vivo, although this gene was strongly inhibited in vitro. CHRM1 was also strongly repressed by androgens in vitro, yet it was found to be stimulated in vivo. OAT, which appears to be downregulated by the AR in vivo, was stimulated in vitro in a ligand independent manner. It remains to be seen whether these inconsistencies result from differential requirements for AR-mediated gene stimulation/repression in vitro and in vivo, the presence of multiple splicing isoforms, or simply erroneous microarray expression scores. Be that as it may, further investigation of genes highlighted by our study, and especially genes for which results from the PCa biopsies are apparently inconsistent with the in vitro results, will have to start with validation of the regulation of such genes in PCa in vivo.

                            Novel AR target genes: potential mechanisms contributing to PCa progression

                            PSA (KLK3) remains the most well studied AR target gene in the PCa literature to date. Since its approval in 1986, serum PSA is routinely used to aid the early diagnosis and prognosis of PCa in men. However, AR-driven PSA expression alone does not fully explain the role of AR in PCa development and progression. Although additional AR target genes have been recently discovered, e.g., FKBP5 [48] and TMPRSS2 [16], most remain elusive. Some of the AR target genes discovered in the present study, and more to be discovered in the future, may open new research avenues and help develop novel therapeutic approaches to manage PCa.


                            Pyrroline-5-carboxylate reductase 1 (PYCR1) catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate (P5C) to proline. Stimulation of PYCR1 by the AR could contribute to PCa progression because P5C is pro-apoptotic [49] and proline is anti-apoptotic [50]. Indeed, a role for PYCR1 in PCa was suggested by a 4-fold increased expression in human prostate tumors compared to adjacent normal tissue [36]. In the present study we demonstrate AR occupancy at the PYCR1 locus in living PCa cells, the functionality of which is suggested by DHT-mediated stimulation of gene expression. Consistent with these in vitro data, we also demonstrate decreased PYCR1 expression in PCa biopsies from men undergoing androgen ablation therapy as compared to untreated controls. The highest PYCR1 expression in our PCa samples was found in biopsies from metastatic tumors, possibly as a result of atypical AR activation. Of the AR targets discovered in the present study, PYCR1 is a strong candidate for mediating the oncogenic action of AR signaling in PCa.


                            Interestingly, another AR target gene discovered in this study also participates in proline metabolism. Ornithine aminotransferase (OAT) converts ornithine to glutamate γ-semialdehyde, which spontaneously cyclizes to form pyrroline-5-carboxylate (P5C), a proline precursor and the substrate for PYCR1. The functional evidence for AR-mediated regulation of OAT is weaker than that for PYCR1. While OAT mRNA was not significantly altered in response to DHT, it was repressed after siRNA-mediated knockdown of the AR, and also displayed a 3.3-fold higher basal expression in C4-2B as compared to LNCaP cells. Interestingly, OAT's expression pattern in our tumor samples does not suggest AR-mediated stimulation, but rather repression, possibly reflecting interactions of AR signaling with input from other cell types or components of the extracellular matrix, which only occur in vivo.


                            In this study, we mapped AR occupancy to a region 0.8-kb upstream of the gene encoding Protein Kinase C delta (PRKCD), which has received much attention in the PCa literature. PRKCD mRNA levels were higher in DHT-treated as compared to untreated PCa cell cultures and were reduced in PCa biopsies from patients undergoing androgen ablation therapy as compared to those from untreated patients. The observed high PRKCD mRNA levels in PCa metastases, which may reflect ligand-independent AR activation, possibly play a role in late stage disease because PRKCD is implicated in growth, migration and invasion of cancer cells, including PCa [51, 52]. PRKCD has also been implicated in the control of cell survival, although most studies suggest it is in fact pro-apoptotic [5355]. Future studies will have to address how PRKCD's pro-apoptotic activity is overcome in advanced PCa cells.

                            CRELD2 and DDT

                            The gene expression data form both the cell culture models and the clinical tumor samples suggest androgen-mediated stimulation of CRELD2 and DDT. Although a role for CRELD2 in carcinogenesis remains to be investigated, this Cysteine-rich with EGF-like Domains 2 (CRELD2) protein has been shown to interact with neuronal acetylcholine receptors [56]. Likewise, no role in tumor progression has been assigned yet to DDT, a protein with homology to macrophage migration inhibitory factor (MIF) [57].


                            Among the few genes near AR-occupied regions that were repressed by DHT was MUC6. Mucin 6 is a secreted glycoprotein that forms a protective gel layer around the producing cell [58]. Other mucins are aberrantly expressed in cancer [58] and MUC2 was ascribed a tumor suppression function [59]. Conceivably, AR-mediated MUC6 repression can contribute to PCa progression. Notably, however, MUC6 mRNA was neither increased in the androgen-ablated compared to the untreated tumors, nor was it absent in the metastatic samples. Although our in vivo data does not support a role for MUC6 in PCa progression, MUC6 could still play a transient role during a short period of time not captured by our clinical samples.

                            TRPV3 and GSTT2

                            Like the androgen-repressed MUC6, evidence for roles for the androgen-stimulated TRPV3 and GSTT2 genes in PCa progression is suggested only from our in vitro data. These two genes are not only androgen stimulated but are also expressed in C4-2B cells more strongly than in LNCaP cells. TRPV3 is a member of the transient receptor potential (TRP) family of thermosensory ion channel genes. Another member of this family, TRPV6, potentiates calcium-dependent cell proliferation [60], and its expression has been linked to human PCa progression [61]. Glutathione S-transferase theta 2 (GSTT2) belongs to a family of detoxification enzymes, overexpression of which is thought to provide cells with protection against oxidative stress and various drugs [62].

                            AR occupancy at PCa-linked loci

                            Two of the AR-occupied regions disclosed by ChIP Display were near loci previously linked to PCa: (i) the hereditary prostate cancer 1 (HPC1) locus, which has been mapped to 1q24-25 [63]; and (ii) the 8q24 locus, recently linked to PCa through admixture mapping in African American men [64]. Although fine mapping of specific genetic elements has not been achieved yet for either of these loci, their contribution to PCa progression in a mechanistic sense may be related to the observed AR occupancy.


                            We have identified 19 novel AR-occupied regions in PCa cells, many of which are associated with genes that are regulated by the AR in either a ligand-dependent or ligand-independent manner. Furthermore, some of the newly identified AR target genes are differentially regulated in cell models for, and/or biopsies from, different stages of PCa progression. These genes provide opportunities for future research to better understand the role of the AR in PCa and eventually improve patient care, especially in the context of castrate-resistant disease.


                            Cell culture and materials

                            Human C4-2B cells, a model for castrate-resistant PCa, were obtained from ViroMed Laboratories Inc. (Minnetonka, MN) and their parental, androgen-dependent LNCaP cells, were obtained from ATCC. (Manassas, VA). The close relationship between the two cell lines was confirmed by microsatellite analysis at ten loci (see Additional file 2). Both C4-2B and LNCaP cells were maintained in RPMI-1640 medium (Invitrogen, Carlsbad, CA) supplemented with 5% fetal bovine serum (FBS; Invitrogen). Dihydrotestosterone (DHT; Sigma Chemical Co., St. Louis, MI) was administered in phenol red-free RPMI-1640 supplemented with 5% charcoal-stripped FBS (CSS; Gemini, West Sacremento, CA). An N-terminal AR antibody (N20) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA).


                            ChIP was carried out essentially as described previously [22]. C4-2B and LNCaP cells were cultured for 3 days in phenol red-free RPMI-1640 supplemented with 5% CSS, then treated for 4-hr with 10 nM DHT, followed by cross-linking with 1% formaldehyde for 10 minutes. After sonication, chromatin was immunoprecipitated overnight at 4°C with either anti-AR antibodies or isotype-matched IgG. AR occupancy was assessed by PCR with locus-specific primers (see Additional file 1) using material from several independent ChIPs. Serial dilutions of genomic DNA were amplified to ensure that PCR was performed within a dynamic range.

                            ChIP Display (CD)

                            We have recently described the CD procedure in detail [19]. Briefly, DNA from AR ChIP and IgG control ChIP was dephosphorylated using shrimp alkaline phosphatase (NEB, Ipswich, MA) and digested with Ava II (NEB). The Ava II fragments were subjected to ligation-mediated PCR using each of 36 combinations of eight primers. Each primer had A or T at the +3 position of the Ava II site, and A,T,G, or C at the so-called +6 position, immediately internal to the Ava II site [19]. In the present paper, primers are named by the nucleotides occupying these two positions. For example, the PCR primer 'AC' is the one with A at the +3 and C at the +6 position. Each PCR reaction in the present study was performed in duplicate, with a 1°C difference in the annealing temperature (see Fig. 1A). The amplified material form 2–3 independent AR ChIPs and 2–3 controls was resolved by polyacrylamide gel electrophoresis (PAGE), and bands enriched in the AR ChIPs were excised and reamplified. They were then subjected to secondary digestion with Hae III, Hinf I and Msp I (NEB), and sub-fragments were isolated by agarose gel electrophoresis and sequenced. The sequences were mapped to the human genome using the SSAHA program on ENSEMBL [65].

                            AR siRNA

                            C4-2B cells (1.5 × 105 cells/well in 6 well plates) were cultured for two days in phenol red-free RPMI-1640 supplemented with 5% CSS. The cells were then transfected using OligofectAMINE (Invitrogen) with 100 nM of either AR-specific or non-specific siRNA (see Additional file 1) as previously described [22]. After two days, cells were treated for 16 hours with 10 nM DHT or ethanol vehicle prior to analysis of gene expression.


                            Cells were grown in six-well plates and RNA was extracted using Biorad's total RNA mini kit according to manufacturer's protocols (Biorad, Hercules, CA). RNA quality was assessed spectrophotometrically and by agarose gel electrophoresis. High quality RNA (200–1000 ng) was reverse-transcribed with random hexamers using the Taqman reverse transcription reagents kit (Applied Biosystems, Foster City, CA). cDNAs of interest were amplified using gene specific-primers (see Additional file 1) and the iQSYBR Green supermix (Biorad). Amplification was performed in triplicate in a 96-well format and monitored in real time using the Opticon 2 DNA Engine (Biorad). Negative controls without RNA, without RT and without cDNA were always included to rule out contamination. Expression levels were determined using standard curves for each gene and corrected for 18S ribosomal RNA levels.

                            Gene expression analysis in clinical PCa specimens

                            Expression of genes disclosed by ChIP Display was analyzed in prostate cancer samples using microarray data collected as part of our previous studies [24]. Briefly, clinical samples were from 40 primary prostate cancers obtained during radical prostatectomy and 7 AR-positive metastatic prostate cancer lesions. Twenty-three of the primary tumors were from patients receiving no therapy before surgery and the remaining 17 were from patients after 3 months of goserelin plus flutamide androgen-ablation therapy. All tissues were obtained during routine clinical management at the Memorial Sloan-Kettering Cancer Center, New York, NY, under protocols approved by the Institutional Review Board. RNA was extracted from manually-microdissected tissue consisting of 60–80% prostate cancer cell nuclei, and analyzed as previously described [24] using the Affymetrix U95 A-E array set. The results are displayed as a heat-map generated using 'Heatmap Builder' (Stanford University), with data routed to 50 equal gates for each probeset (row) using a linear grey scale gradient from white (lowest value) to black (highest value). Data for each gene was generated using only those high-fidelity probesets with a grade A annotation as defined by Affymetrix.

                            Statistical analysis

                            We employed the unpaired t-test using GraphPad Instat version 3.0 for PC to compare mRNA levels for each gene between DHT-treated and vehicle-treated cells at each time point, and between the basal levels in LNCaP versus C4-2B cells. Unless otherwise stated, differences referred to in the text were assigned a p value of less than 0.05. The individual p values assigned to each of the comparisons are provided in additional files 3 and 4.



                            This work was supported by the following grants: W81XWH-05-1-0025 from the Department of Defense (to BF), CA109147 (to GAC) and DK071122 (to BF) from the National Institutes of Health, 299048 & 453662 from the National Health and Medical Research Council of Australia (to WDT, ID#453662) P50 CA92629 and MSKCC SPORE in prostate cancer from the National Institutes of Health, a grant from PepsiCo Foundation (to HIS), grants from the Prostate Cancer Foundation (to HIS and GAC). GB is a recipient of a National Health and Medical Research Council of Australia CJ Martin Biomedical Fellowship. SP was partially supported by NIH training grant T 32 GM067587. BF holds the J. Harold and Edna L. LaBriola Chair in Genetic Orthopaedic Research at the University of Southern California. The experiments were conducted in a facility constructed with support from Research Facilities Improvement Program Grant Number C06 (RR10600-01, CA62528-01, RR14514-01) from the NIH/NCRR.

                            Dr. David Van Den Berg (Norris Comprehensive Cancer Centre, Genomics Core Facility, University of Southern California, Los Angeles, CA, USA) provided technical support for genotype analysis.

                            Authors’ Affiliations

                            Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California
                            Department of Preventive Medicine, Keck School of Medicine, University of Southern California
                            Department of Urology, Keck School of Medicine, University of Southern California
                            Department of Orthopedic Surgery, Keck School of Medicine, University of Southern California
                            Dame Roma Mitchell Cancer Research Laboratories, School of Medicine, The University of Adelaide/Hanson Institute
                            Genitourinary Oncology Service, Division of Solid Tumor Oncology, Memorial Sloan-Kettering Cancer Center, Department of Medicine, Joan and Sanford I. Weill College of Medicine


                            1. Hsing AW, Chokkalingam AP: Prostate cancer epidemiology. Front Biosci. 2006, 11: 1388-1413. 10.2741/1891View ArticlePubMed
                            2. Marker PC, Donjacour AA, Dahiya R, Cunha GR: Hormonal, cellular, and molecular control of prostatic development. Dev Biol. 2003, 253 (2): 165-174. 10.1016/S0012-1606(02)00031-3View ArticlePubMed
                            3. Bonkhoff H, Remberger K: Differentiation pathways and histogenetic aspects of normal and abnormal prostatic growth: a stem cell model. Prostate. 1996, 28 (2): 98-106. 10.1002/(SICI)1097-0045(199602)28:2<98::AID-PROS4>3.0.CO;2-JView ArticlePubMed
                            4. Geck P, Szelei J, Jimenez J, Lin TM, Sonnenschein C, Soto AM: Expression of novel genes linked to the androgen-induced, proliferative shutoff in prostate cancer cells. J Steroid Biochem Mol Biol. 1997, 63 (4–6): 211-218. 10.1016/S0960-0760(97)00122-2View ArticlePubMed
                            5. Scher HI, Buchanan G, Gerald W, Butler LM, Tilley WD: Targeting the androgen receptor: improving outcomes for castration-resistant prostate cancer. Endocr Relat Cancer. 2004, 11 (3): 459-476. 10.1677/erc.1.00525View ArticlePubMed
                            6. Balk SP: Androgen receptor as a target in androgen-independent prostate cancer. Urology. 2002, 60 (3 Suppl 1): 132-138. discussion 138–139., 10.1016/S0090-4295(02)01593-5View ArticlePubMed
                            7. Buchanan G, Irvine RA, Coetzee GA, Tilley WD: Contribution of the androgen receptor to prostate cancer predisposition and progression. Cancer Metastasis Rev. 2001, 20 (3–4): 207-223. 10.1023/A:1015531326689View ArticlePubMed
                            8. Zegarra-Moro OL, Schmidt LJ, Huang H, Tindall DJ: Disruption of androgen receptor function inhibits proliferation of androgen-refractory prostate cancer cells. Cancer Res. 2002, 62 (4): 1008-1013.PubMed
                            9. Chen CD, Welsbie DS, Tran C, Baek SH, Chen R, Vessella R, Rosenfeld MG, Sawyers CL: Molecular determinants of resistance to antiandrogen therapy. Nat Med. 2004, 10 (1): 33-39. 10.1038/nm972View ArticlePubMed
                            10. Han G, Buchanan G, Ittmann M, Harris JM, Yu X, Demayo FJ, Tilley W, Greenberg NM: Mutation of the androgen receptor causes oncogenic transformation of the prostate. Proc Natl Acad Sci USA. 2005, 102 (4): 1151-1156. 10.1073/pnas.0408925102PubMed CentralView ArticlePubMed
                            11. Borgono CA, Diamandis EP: The emerging roles of human tissue kallikreins in cancer. Nat Rev Cancer. 2004, 4 (11): 876-890. 10.1038/nrc1474View ArticlePubMed
                            12. Whitbread AK, Veveris-Lowe TL, Lawrence MG, Nicol DL, Clements JA: The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition. Biol Chem. 2006, 387 (6): 707-714. 10.1515/BC.2006.089View ArticlePubMed
                            13. Gnanapragasam VJ, Robson CN, Neal DE, Leung HY: Regulation of FGF8 expression by the androgen receptor in human prostate cancer. Oncogene. 2002, 21 (33): 5069-5080. 10.1038/sj.onc.1205663View ArticlePubMed
                            14. Gregory CW, Hamil KG, Kim D, Hall SH, Pretlow TG, Mohler JL, French FS: Androgen receptor expression in androgen-independent prostate cancer is associated with increased expression of androgen-regulated genes. Cancer Res. 1998, 58 (24): 5718-5724.PubMed
                            15. Xu LL, Shi Y, Petrovics G, Sun C, Makarem M, Zhang W, Sesterhenn IA, McLeod DG, Sun L, Moul JW: PMEPA1, an androgen-regulated NEDD4-binding protein, exhibits cell growth inhibitory function and decreased expression during prostate cancer progression. Cancer Res. 2003, 63 (15): 4299-4304.PubMed
                            16. Lin B, Ferguson C, White JT, Wang S, Vessella R, True LD, Hood L, Nelson PS: Prostate-localized and androgen-regulated expression of the membrane-bound serine protease TMPRSS2. Cancer Res. 1999, 59 (17): 4180-4184.PubMed
                            17. Tomlins SA, Mehra R, Rhodes DR, Smith LR, Roulston D, Helgeson BE, Cao X, Wei JT, Rubin MA, Shah RB: TMPRSS2:ETV4 gene fusions define a third molecular subtype of prostate cancer. Cancer Res. 2006, 66 (7): 3396-3400. 10.1158/0008-5472.CAN-06-0168View ArticlePubMed
                            18. Tomlins SA, Rhodes DR, Perner S, Dhanasekaran SM, Mehra R, Sun XW, Varambally S, Cao X, Tchinda J, Kuefer R: Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science. 2005, 310 (5748): 644-648. 10.1126/science.1117679View ArticlePubMed
                            19. Barski A, Frenkel B: ChIP Display: novel method for identification of genomic targets of transcription factors. Nucleic Acids Res. 2004, 32 (12): e104- 10.1093/nar/gnh097PubMed CentralView ArticlePubMed
                            20. Thalmann GN, Anezinis PE, Chang SM, Zhau HE, Kim EE, Hopwood VL, Pathak S, von Eschenbach AC, Chung LW: Androgen-independent cancer progression and bone metastasis in the LNCaP model of human prostate cancer. Cancer Res. 1994, 54 (10): 2577-2581.PubMed
                            21. Prescott J, Jariwala U, Jia L, Cogan JP, Barski A, Pregizer S, Arasheben A, Neilson JJ, Frenkel B, Coetzee GA: Androgen Receptor-Mediated Repression of Novel Target Genes. The Prostate. 2007, ,
                            22. Jia L, Shen HC, Wantroba M, Khalid O, Liang G, Wang Q, Gentzschein E, Pinski JK, Stanczyk FZ, Jones PA: Locus-wide chromatin remodeling and enhanced androgen receptor-mediated transcription in recurrent prostate tumor cells. Mol Cell Biol. 2006, 26 (19): 7331-7341. 10.1128/MCB.00581-06PubMed CentralView ArticlePubMed
                            23. Jia L, Kim J, Shen H, Clark PE, Tilley WD, Coetzee GA: Androgen receptor activity at the prostate specific antigen locus: steroidal and non-steroidal mechanisms. Mol Cancer Res. 2003, 1 (5): 385-392.PubMed
                            24. Holzbeierlein J, Lal P, LaTulippe E, Smith A, Satagopan J, Zhang L, Ryan C, Smith S, Scher H, Scardino P: Gene expression analysis of human prostate carcinoma during hormonal therapy identifies androgen-responsive genes and mechanisms of therapy resistance. Am J Pathol. 2004, 164 (1): 217-227.PubMed CentralView ArticlePubMed
                            25. Scher HI, Sawyers CL: Biology of progressive, castration-resistant prostate cancer: directed therapies targeting the androgen-receptor signaling axis. J Clin Oncol. 2005, 23 (32): 8253-8261. 10.1200/JCO.2005.03.4777View ArticlePubMed
                            26. Rennie PS, Bruchovsky N, Leco KJ, Sheppard PC, McQueen SA, Cheng H, Snoek R, Hamel A, Bock ME, MacDonald BS: Characterization of two cis-acting DNA elements involved in the androgen regulation of the probasin gene. Mol Endocrinol. 1993, 7 (1): 23-36. 10.1210/me.7.1.23PubMed
                            27. Murtha P, Tindall DJ, Young CY: Androgen induction of a human prostate-specific kallikrein, hKLK2: characterization of an androgen response element in the 5' promoter region of the gene. Biochemistry. 1993, 32 (25): 6459-6464. 10.1021/bi00076a020View ArticlePubMed
                            28. Riegman PH, Vlietstra RJ, van der Korput JA, Brinkmann AO, Trapman J: The promoter of the prostate-specific antigen gene contains a functional androgen responsive element. Mol Endocrinol. 1991, 5 (12): 1921-1930.View ArticlePubMed
                            29. Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF: Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006, 38 (11): 1289-1297. 10.1038/ng1901View ArticlePubMed
                            30. Wei CL, Wu Q, Vega VB, Chiu KP, Ng P, Zhang T, Shahab A, Yong HC, Fu Y, Weng Z: A global map of p53 transcription-factor binding sites in the human genome. Cell. 2006, 124 (1): 207-219. 10.1016/j.cell.2005.10.043View ArticlePubMed
                            31. Wang Q, Carroll JS, Brown M: Spatial and temporal recruitment of androgen receptor and its coactivators involves chromosomal looping and polymerase tracking. Mol Cell. 2005, 19 (5): 631-642. 10.1016/j.molcel.2005.07.018View ArticlePubMed
                            32. Spilianakis CG, Lalioti MD, Town T, Lee GR, Flavell RA: Interchromosomal associations between alternatively expressed loci. Nature. 2005, 435 (7042): 637-645. 10.1038/nature03574View ArticlePubMed
                            33. Willingham AT, Gingeras TR: TUF love for "junk" DNA. Cell. 2006, 125 (7): 1215-1220. 10.1016/j.cell.2006.06.009View ArticlePubMed
                            34. Nelson PS, Clegg N, Arnold H, Ferguson C, Bonham M, White J, Hood L, Lin B: The program of androgen-responsive genes in neoplastic prostate epithelium. Proc Natl Acad Sci USA. 2002, 99 (18): 11890-11895. 10.1073/pnas.182376299PubMed CentralView ArticlePubMed
                            35. Xu LL, Su YP, Labiche R, Segawa T, Shanmugam N, McLeod DG, Moul JW, Srivastava S: Quantitative expression profile of androgen-regulated genes in prostate cancer cells and identification of prostate-specific genes. Int J Cancer. 2001, 92 (3): 322-328. 10.1002/ijc.1196View ArticlePubMed
                            36. Ernst T, Hergenhahn M, Kenzelmann M, Cohen CD, Bonrouhi M, Weninger A, Klaren R, Grone EF, Wiesel M, Gudemann C: Decrease and gain of gene expression are equally discriminatory markers for prostate carcinoma: a gene expression analysis on total and microdissected prostate tissue. Am J Pathol. 2002, 160 (6): 2169-2180.PubMed CentralView ArticlePubMed
                            37. Cawley S, Bekiranov S, Ng HH, Kapranov P, Sekinger EA, Kampa D, Piccolboni A, Sementchenko V, Cheng J, Williams AJ: Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs. Cell. 2004, 116 (4): 499-509. 10.1016/S0092-8674(04)00127-8View ArticlePubMed
                            38. Horak CE, Snyder M: ChIP-chip: a genomic approach for identifying transcription factor binding sites. Methods Enzymol. 2002, 350: 469-483.View ArticlePubMed
                            39. Ren B, Robert F, Wyrick JJ, Aparicio O, Jennings EG, Simon I, Zeitlinger J, Schreiber J, Hannett N, Kanin E: Genome-wide location and function of DNA binding proteins. Science. 2000, 290 (5500): 2306-2309. 10.1126/science.290.5500.2306View ArticlePubMed
                            40. Chen J, Sadowski I: Identification of the mismatch repair genes PMS2 and MLH1 as p53 target genes by using serial analysis of binding elements. Proc Natl Acad Sci USA. 2005, 102 (13): 4813-4818. 10.1073/pnas.0407069102PubMed CentralView ArticlePubMed
                            41. Kim J, Bhinge AA, Morgan XC, Iyer VR: Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment. Nat Methods. 2005, 2 (1): 47-53. 10.1038/nmeth726View ArticlePubMed
                            42. Roh TY, Ngau WC, Cui K, Landsman D, Zhao K: High-resolution genome-wide mapping of histone modifications. Nat Biotechnol. 2004, 22 (8): 1013-1016. 10.1038/nbt990View ArticlePubMed
                            43. Impey S, McCorkle SR, Cha-Molstad H, Dwyer JM, Yochum GS, Boss JM, McWeeney S, Dunn JJ, Mandel G, Goodman RH: Defining the CREB regulon: a genome-wide analysis of transcription factor regulatory regions. Cell. 2004, 119 (7): 1041-1054.PubMed
                            44. van Steensel B, Henikoff S: Identification of in vivo DNA targets of chromatin proteins using tethered dam methyltransferase. Nat Biotechnol. 2000, 18 (4): 424-428. 10.1038/74487View ArticlePubMed
                            45. Haag P, Bektic J, Bartsch G, Klocker H, Eder IE: Androgen receptor down regulation by small interference RNA induces cell growth inhibition in androgen sensitive as well as in androgen independent prostate cancer cells. J Steroid Biochem Mol Biol. 2005, 96 (3–4): 251-258. 10.1016/j.jsbmb.2005.04.029View ArticlePubMed
                            46. Chang HC, Chen SC, Chen J, Hsieh JT: In vitro gene expression changes of androgen receptor coactivators after hormone deprivation in an androgen-dependent prostate cancer cell line. J Formos Med Assoc. 2005, 104 (9): 652-658.PubMed
                            47. Li P, Yu X, Ge K, Melamed J, Roeder RG, Wang Z: Heterogeneous expression and functions of androgen receptor co-factors in primary prostate cancer. Am J Pathol. 2002, 161 (4): 1467-1474.PubMed CentralView ArticlePubMed
                            48. Magee JA, Chang LW, Stormo GD, Milbrandt J: Direct, androgen receptor-mediated regulation of the FKBP5 gene via a distal enhancer element. Endocrinology. 2006, 147 (1): 590-598. 10.1210/en.2005-1001View ArticlePubMed
                            49. Maxwell SA, Davis GE: Differential gene expression in p53-mediated apoptosis-resistant vs. apoptosis-sensitive tumor cell lines. Proc Natl Acad Sci USA. 2000, 97 (24): 13009-13014. 10.1073/pnas.230445997PubMed CentralView ArticlePubMed
                            50. Chen C, Dickman MB: Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolii. Proc Natl Acad Sci USA. 2005, 102 (9): 3459-3464. 10.1073/pnas.0407960102PubMed CentralView ArticlePubMed
                            51. Kiley SC, Clark KJ, Goodnough M, Welch DR, Jaken S: Protein kinase C delta involvement in mammary tumor cell metastasis. Cancer Res. 1999, 59 (13): 3230-3238.PubMed
                            52. Kharait S, Dhir R, Lauffenburger D, Wells A: Protein kinase Cdelta signaling downstream of the EGF receptor mediates migration and invasiveness of prostate cancer cells. Biochem Biophys Res Commun. 2006, 343 (3): 848-856.View ArticlePubMed
                            53. Fujii T, Garcia-Bermejo ML, Bernabo JL, Caamano J, Ohba M, Kuroki T, Li L, Yuspa SH, Kazanietz MG: Involvement of protein kinase C delta (PKCdelta) in phorbol ester-induced apoptosis in LNCaP prostate cancer cells. Lack of proteolytic cleavage of PKCdelta. J Biol Chem. 2000, 275 (11): 7574-7582. 10.1074/jbc.275.11.7574View ArticlePubMed
                            54. Sumitomo M, Ohba M, Asakuma J, Asano T, Kuroki T, Asano T, Hayakawa M: Protein kinase Cdelta amplifies ceramide formation via mitochondrial signaling in prostate cancer cells. J Clin Invest. 2002, 109 (6): 827-836. 10.1172/JCI200214146PubMed CentralView ArticlePubMed
                            55. Tanaka Y, Gavrielides MV, Mitsuuchi Y, Fujii T, Kazanietz MG: Protein kinase C promotes apoptosis in LNCaP prostate cancer cells through activation of p38 MAPK and inhibition of the Akt survival pathway. J Biol Chem. 2003, 278 (36): 33753-33762. 10.1074/jbc.M303313200View ArticlePubMed
                            56. Ortiz JA, Castillo M, del Toro ED, Mulet J, Gerber S, Valor LM, Sala S, Sala F, Gutierrez LM, Criado M: The cysteine-rich with EGF-like domains 2 (CRELD2) protein interacts with the large cytoplasmic domain of human neuronal nicotinic acetylcholine receptor alpha4 and beta2 subunits. J Neurochem. 2005, 95 (6): 1585-1596. 10.1111/j.1471-4159.2005.03473.xView ArticlePubMed
                            57. Nishihira J, Fujinaga M, Kuriyama T, Suzuki M, Sugimoto H, Nakagawa A, Tanaka I, Sakai M: Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for enzyme activation. Biochem Biophys Res Commun. 1998, 243 (2): 538-544. 10.1006/bbrc.1998.8123View ArticlePubMed
                            58. Hollingsworth MA, Swanson BJ: Mucins in cancer: protection and control of the cell surface. Nat Rev Cancer. 2004, 4 (1): 45-60. 10.1038/nrc1251View ArticlePubMed
                            59. Velcich A, Yang W, Heyer J, Fragale A, Nicholas C, Viani S, Kucherlapati R, Lipkin M, Yang K, Augenlicht L: Colorectal cancer in mice genetically deficient in the mucin Muc2. Science. 2002, 295 (5560): 1726-1729. 10.1126/science.1069094View ArticlePubMed
                            60. Schwarz EC, Wissenbach U, Niemeyer BA, Strauss B, Philipp SE, Flockerzi V, Hoth M: TRPV6 potentiates calcium-dependent cell proliferation. Cell Calcium. 2006, 39 (2): 163-173. 10.1016/j.ceca.2005.10.006View ArticlePubMed
                            61. Fixemer T, Wissenbach U, Flockerzi V, Bonkhoff H: Expression of the Ca2+-selective cation channel TRPV6 in human prostate cancer: a novel prognostic marker for tumor progression. Oncogene. 2003, 22 (49): 7858-7861. 10.1038/sj.onc.1206895View ArticlePubMed
                            62. Hayes JD, Pulford DJ: The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol. 1995, 30 (6): 445-600.View ArticlePubMed
                            63. Xu J, Zheng SL, Chang B, Smith JR, Carpten JD, Stine OC, Isaacs SD, Wiley KE, Henning L, Ewing C: Linkage of prostate cancer susceptibility loci to chromosome 1. Hum Genet. 2001, 108 (4): 335-345. 10.1007/s004390100488View ArticlePubMed
                            64. Freedman ML, Haiman CA, Patterson N, McDonald GJ, Tandon A, Waliszewska A, Penney K, Steen RG, Ardlie K, John EM: Admixture mapping identifies 8q24 as a prostate cancer risk locus in African-American men. Proc Natl Acad Sci USA. 2006, 103 (38): 14068-14073. 10.1073/pnas.0605832103PubMed CentralView ArticlePubMed
                            65. Ensembl: [http://​www.​ensembl.​org]
                            66. Consite: [http://​www.​phylofoot.​org/​consite]
                            67. Ouyang X, DeWeese TL, Nelson WG, Abate-Shen C: Loss-of-function of Nkx3.1 promotes increased oxidative damage in prostate carcinogenesis. Cancer Res. 2005, 65 (15): 6773-6779. 10.1158/0008-5472.CAN-05-1948View ArticlePubMed
                            68. Yamaguchi M, Yamamoto K, Miura O: Aberrant expression of the LHX4 LIM-homeobox gene caused by t(1;14)(q25;q32) in chronic myelogenous leukemia in biphenotypic blast crisis. Genes Chromosomes Cancer. 2003, 38 (3): 269-273. 10.1002/gcc.10283View ArticlePubMed
                            69. Verras M, Sun Z: Roles and regulation of Wnt signaling and beta-catenin in prostate cancer. Cancer Lett. 2006, 237 (1): 22-32. 10.1016/j.canlet.2005.06.004View ArticlePubMed
                            70. Hewitt KJ, Agarwal R, Morin PJ: The claudin gene family: expression in normal and neoplastic tissues. BMC Cancer. 2006, 6: 186- 10.1186/1471-2407-6-186PubMed CentralView ArticlePubMed
                            71. Ashraf N, Zino S, Macintyre A, Kingsmore D, Payne AP, George WD, Shiels PG: Altered sirtuin expression is associated with node-positive breast cancer. Br J Cancer. 2006, 95 (8): 1056-1061. 10.1038/sj.bjc.6603384PubMed CentralView ArticlePubMed
                            72. Langley E, Pearson M, Faretta M, Bauer UM, Frye RA, Minucci S, Pelicci PG, Kouzarides T: Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence. Embo J. 2002, 21 (10): 2383-2396. 10.1093/emboj/21.10.2383PubMed CentralView ArticlePubMed

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