Myeloproliferative disorder FOP-FGFR1 fusion kinase recruits phosphoinositide-3 kinase and phospholipase Cγ at the centrosome
© Lelièvre et al; licensee BioMed Central Ltd. 2008
Received: 04 March 2008
Accepted: 15 April 2008
Published: 15 April 2008
The t(6;8) translocation found in rare and agressive myeloproliferative disorders results in a chimeric gene encoding the FOP-FGFR1 fusion protein. This protein comprises the N-terminal region of the centrosomal protein FOP and the tyrosine kinase of the FGFR1 receptor. FOP-FGFR1 is localized at the centrosome where it exerts a constitutive kinase activity.
We show that FOP-FGFR1 interacts with the large centrosomal protein CAP350 and that CAP350 is necessary for FOP-FGFR1 localisation at centrosome. FOP-FGFR1 activates the phosphoinositide-3 kinase (PI3K) pathway. We show that p85 interacts with tyrosine 475 of FOP-FGFR1, which is located in a YXXM consensus binding sequence for an SH2 domain of p85. This interaction is in part responsible for PI3K activation. Ba/F3 cells that express FOP-FGFR1 mutated at tyrosine 475 have reduced proliferative ability. Treatment with PI3K pathway inhibitors induces death of FOP-FGFR1 expressing cells. FOP-FGFR1 also recruits phospholipase Cγ1 (PLCγ1) at the centrosome. We show that this enzyme is recruited by FOP-FGFR1 at the centrosome during interphase.
These results delineate a particular type of oncogenic mechanism by which an ectopic kinase recruits its substrates at the centrosome whence unappropriate signaling induces continuous cell growth and MPD.
The FGFR1 gene, located at 8p12, encodes a tyrosine kinase receptor for members of the FGF family . Chromosomal rearrangements that affect FGFR1 induce an atypical myeloproliferative disorder (MPD), characterized by dual lympho and myeloproliferation and aggressive evolution. In this MPD, the FGFR1 tyrosine kinase is fused to one of several partners, including BCR , CEP110 , ERVK , FOP (FGFR1 oncogene partner) , MYO18A , TIF1  and ZNF198 .
The FOP-FGFR1 fusion protein, in which the N-terminal FOP protein-protein interaction sequence is fused to the tyrosine kinase region of FGFR1, is encoded by a chimeric gene that results from a translocation between chromosomal regions 8p12 and 6q27. The FOP moiety mediates dimerization of the fusion kinase whose constitutive activity triggers downstream signaling pathways including the phosphoinositide-3 kinase (PI3K) pathway . PI3K is a heterodimer comprising a p85 regulatory subunit and a p110 catalytic subunit that catalyzes the phosphorylation of inositol lipids from the plasma membrane. PI3K can be activated by interaction of p85 with a phosphorylated tyrosine in a YXXM motif, a consensus binding amino acid sequence for the SH2 domains of p85 . FOP-FGFR1 also binds and activates PLCγ1 .
The transmembrane region of FGFR1 is not conserved in the fusion protein, which is thus unhooked from the membrane. FOP is a centrosomal protein . It interacts with the centrosomal protein CAP350 . FOP-FGFR1 is also addressed to the centrosome  where it induces phosphorylations on tyrosine residues. Other partner proteins in fusion kinases, such as CEP110, NIN, PDE4DIP, PCM1 and TRIP11 are also centrosomal proteins . The centrosome is a small organelle that control several cell processes including microtubules organization and cell cycle progression. Many cell cycle regulatory molecules are localized at the centrosome [11, 15]. Localization at the centrosome may help find or recruit appropriate substrates to induce cell survival and proliferation explaining the oncogenicity of the to the fusion kinase. These substrates could either be signaling molecules phosphorylated at the centrosome or intrinsic centrosomal proteins.
In this report, we have characterized the interaction of FOP-FGFR1 with CAP350, and shown that the localization of the tyrosine kinase at the centrosome induces the recruitment in this specific area of two enzymatic substrates, PI3K and PLCγ1.
Plasmids, cells and reagents
Ba/F3 cells hematopoietic cells were grown as described . HeLa and Cos-1 cells were grown in DMEM with 10% fetal calf serum (FCS). Myc-tagged FOP-FGFR1 and myc-tagged FOP-FGFR1 kinase defective (K259A) and corresponding clones of stably-transfected Ba/F3 cells have been previously described [3, 5, 9]. Ba/F3 stably expressing BCR-ABL or the EPO receptor (EPO-R) are a gift from P. Dubreuil. GFP-tagged FOP-FGFR1 was obtained by insertion in pEGFP vector conferring GFP tag at the N-terminus. The quickchange site-directed mutagenesis kit from Stratagene (La Jolla, CA) was used to introduce point mutations changing tyrosine to phenylalanine at position 511 and 475 of FOP-FGFR1 (Y511F, Y475F, and both for DBL mutant). Each construct was verified by sequencing. Myc-tagged CAP350 (AA 1–3117) and myc-tagged CAP350 C-terminal domain (AA 1896–3117) constructs were obtained by subcloning in pCS2+ with a myc tag (pMT plasmid). The plasmid which direct the GST-SH2 N-terminal p85α has been described . Transient and stable transfections were done as described .
The following sequences were used: siCAP1, 5'-AUGAACGAUAUCAGUGCUAUAUU-3' , siCAP2, 5'-CAGGUAGUAGUCAUCUUAUAAUU-3' , siControl, non targeting siRNA (Dharmacon, D-001210-01). siRNA duplexes were synthesized by Dharmacon (Lafayette, USA) and RNAi experiments were done as previously described .
We used monoclonal anti-myc (9E10), polyclonal anti-FGFR1 (C-15), and polyclonal anti-PLCγ1 (1249), from Santa Cruz Biotechology (Heidelberg, Germany), polyclonal anti-p85 (06–195) from Upstate Biotechnology (Lake Placid, NY), monoclonal anti-p85 (ab249) and anti-GFP (ab290) from Abcam (Paris, France), polyclonal anti-pYXXM (3821) and polyclonal anti-pPLCγ1 (2821) from Cell Signaling (Danvers, MA), anti-γ-tubulin either monoclonal (GTU-88) or polyclonal (T3559) from Sigma-Aldrich (Lyon, France). Polyclonal anti-CAP350 antibody was obtained by immunizing rabbits against the AA 1875–2055 domain of CAP350.
Cell lysis, immunoprecipitations, western blot and GST pull-down
Ba/F3 cells were starved over 7 h in RPMI containing 0.5% FCS. For immunoprecipitation assays, cells were lysed in NP40 1% lysis buffer. Protein extracts were incubated with antibodies and subsequently with either protein A- or G-agarose. Bound proteins or total protein extract were separated as described  or using NuPAGE 4–12% Novex Bis-Tris gels according to the manufacturer's instructions (Invitrogen, Cergy Pontoise, France) for CAP350 experiments. For GST pull-down experiments, 10 μg of bacterially product GST-p85 or GST alone were used as previously described .
Cell survival assays
1.106 Ba/F3 cells were washed three times and grown in triplicate in the absence of IL3. The number of viable cells was measured by trypan blue exclusion. For PI3K inhibition experiments, 10 μM LY294002 (Sigma-Aldrich) was used.
Immunofluorescence and confocal analysis
HeLa cells were grown on coverslips and starved Ba/F3 cells were centrifugated to adhere to the coverslips. Cells were fixed for 6 min using methanol, at -20°C. Afters washes, cells were sequentially incubated with primary antibodies and with Cy2, Cy3 and Cy5 coupled anti-rabbit or anti-mouse secondary antibodies (Jackson ImmunoResearch Laboratories, Suffolk, UK). DNA was stained using DAPI (Sigma-Aldrich). Preparation were analyzed with Zeiss Laser Scanning confocal microscope LSM510. Images were acquired using 63X objective and Z-series were projected onto a single view. Images were processed using Photoshop 8.0.1.
FOP-FGFR1 fusion protein interacts with CAP350 at the centrosome
CAP350 depletion prevents FOP-FGFR1 centrosomal localization
CAP350 was depleted using two different oligonucleotides duplexes (siCAP1 and siCAP2). Depletion efficiency was demonstrated by both western blotting (Figure 1C) and immunofluorescence (Figure 1D). CAP350 depletion abolished the association of FOP-FGFR1 with centrosomes in interphase (Figure 1D) and M phase cells (not shown).
FOP-FGFR1 recruits the p85 subunit of PI3K at the centrosome during interphase
FOP-FGFR1 interacts with the p85 subunit of the PI3K and provides a consensus binding motif at the centrosome
We next tested whether FOP-FGFR1 and p85 interact in vivo. Using anti-FGFR1 antibody we immunoprecipitated lysates from wild-type Ba/F3 cells or Ba/F3 cells stably transfected with FOP-FGFR1. Western-blot analysis with anti-p85 antibody showed that FOP-FGFR1 interacts with p85 (Figure 2B). The p85 subunit preferentially binds a phosphorylated tyrosine in a YXXM motif. Immunofluorescence experiments with an antibody recognizing this phosphorylated motif showed that FOP-FGFR1 provides a consensus binding site for p85 at the centrosome (Figure 2Cd). Neither K259A mutant-expressing cells nor wild-type Ba/F3 cells showed the same result (Figure 2Ca,g). The fact that p85 and the phosphorylated motif for its activation are concentrated at the centrosome suggests that PI3K is activated at the centrosome in FOP-FGFR1-expressing cells.
FOP-FGFR1 interacts with p85 through its tyrosine 475
Mutation of tyrosine 475 only partially reduces p85 recruitment at the centrosome
Since mutation of tyrosine 475 reduces FOP-FGFR1 interaction with p85, we investigated whether the FOP-FGFR1 Y475F fusion protein could still induce recruitment of p85 at the centrosome. We confirmed that all our FOP-FGFR1 mutants localized at the centrosome, and showed that recruitment of p85 at the centrosome was partially reduced in FOP-FGFR1 Y475F cells (Figure 3Cf). Surprisingly, it was also reduced in Y511F cells, which lacks the PLCγ binding site (Figure 3Cj) and in DBL MUT cells (Figure 3Cn). These results indicate that the recruitment of p85 is not solely due to the interaction through tyrosine 475 but implicate other sites such as tyrosine 511. Mutation of FOP-FGFR1 on tyrosine 475 did not abolish the pYXXM staining at the centrosome, although western-blot analysis showed that the FOP-FGFR1 Y475F protein was no longer phosphorylated on this motif (Figure 3E, D). This suggests that other tyrosine residues, in particular tyrosine 511, which is not in a PI3K consensus binding motif, indirectly interact with p85 through adaptor molecules and provide a phosphotyrosine in a YXXM motif at the centrosome.
FOP-FGFR1 induces cell proliferation and survival in a PI3K dependent manner
FOP-FGFR1 recruits and activates PLCγ1 at the centrosome
We have shown here that the FOP-FGFR1 oncogenic tyrosine kinase encounters or recruits different partners at the centrosome. We first showed that FOP-FGFR1 interacts with CAP350. CAP350 is essential for FOP localization at the centrosome . CAP350 is a large centrosomal protein with many coiled-coil motifs . It is phosphorylated during mitosis and has an ATP/GTP-binding site motif. Little is known about CAP350 functions at the centrosome. CAP350 and FOP form a complex required for anchoring microtubules at the centrosome . CAP350 could serve as a scaffold for anchoring a large number of regulatory molecules, including an ectopic fusion protein. The presence of a constitutive tyrosine kinase activity on this platform could alter the function of centrosomal proteins involved in cell cycle regulation, including CAP350 itself. It is not known whether CAP350 also interacts with CEP110, the centrosomal partner of the CEP110-FGFR1 fusion kinase found in MPD with t(8;9). However, our yeast two-hybrid screens does not suggest it does (data not shown).
We next showed that FOP-FGFR1 interacts with p85 and that the mutation of tyrosine 475 in the FOP-FGFR1 kinase domain reduces this interaction. The corresponding tyrosine in the FGFR1 sequence is tyrosine 730 which has never been described as phosphorylated  and for which no interacting substrate has been reported. The PI3K pathway is involved whenever FGFR1 is stimulated. PI3K seems to be activated mainly through the interaction with the FGFR1 juxtamembrane domain, due to adaptor proteins providing the consensus binding motif for p85, such as GAB1 [19, 20]. However, this domain is disrupted in FOP-FGFR1. Our results suggest that tyrosine 475 is phosphorylated in the FOP-FGFR1 protein and provides a de novo binding motif for p85 allowing PI3K interaction and activation. We propose that it is the sustained kinase activity of FOP-FGFR1 compared to the physiologic intermittent activation of FGFR1, which allows tyrosine 475 phosphorylation. However, this interaction is only one way to PI3K activation. Other tyrosines, such as Y511, must be involved in an indirect interaction with p85. Tyrosine 511 could indirectly interact with p85 through adaptor molecules such as SHC. Another possibility is that FOP protein partners such as CAP350 or others may somehow participate in recruiting PI3K.
PI3K is usually associated with the plasma membrane, downstream of tyrosine kinases. We show here that p85 can be recruited at the centrosome upon oncogenic signaling. This may also occur in physiological conditions. Stimulation of cells expressing the insulin receptor with insulin triggers the association of PI3K with γ-tubulin, suggesting that PI3K is recruited at the centrosome . The presence of the p85 subunit at the centrosome suggests that lipid products might be concentrated in this structure. Although large cytosolic pools of PI3K have been described [22, 23], PI3K activity should eventually involve interaction with a membrane, since both the substrate and products of PI3K are membrane constituents. Under stimulation of the insulin receptor, PI3K is associated with intracellular membranes and to a lesser extent with the plasma membrane . Since the Golgi aparatus is connected to the centrosome, it is possible that centrosomal PI3K associates with vesicles derived from the Golgi membranes. Because p85 recruitment by FOP-FGFR1 is not strictly restricted to the centrosome, it is also possible that p85 localizes in the centrosome/Golgi area.
The role of PI3K at the centrosome remains to be determined. We have previously demonstrated that FOP-FGFR1 induces continuous entry in S phase [13, 14]. Another study has suggested that PI3K is required for centrosome duplication and/or separation, which occurs during the G1 and S phase . The CDK2-cyclin E complex is required for both DNA replication and centrosome duplication [26, 27], and PI3K can enhance phosphorylation and activation of CDK2 . Thus, PI3K activated at the centrosome by FOP-FGFR1 could alter the phosphorylation level of centrosomal components such as CDK2 and trigger centrosome duplication in G1/S phase of the cell cycle.
FOP-FGFR1 activates and recruits PLCγ1 at the centrosome. Mutation of Y511 PLCγ1-binding site affects cell survival and proliferation and binding of PCLγ1. This suggests that PLCγ interaction and activation is also important for proliferation and survival of FOP-FGFR1-expressing Ba/F3 cells and that PI3K and PLCγ1 might act synergistically. This is in agreement with the fact that Y511F mutant FOP-FGFR1 fails to recruit p85 at the centrosome. PI3K and PLCγ1 share common downstream substrates such as phosphatidylinositol-4,5-biphosphate (PIP2) and protein kinase C (PKC). An isoform of PKC, PKCε, localizes at the centrosome in a hypophosphorylated state . Interestingly, both downtream effectors of PI3K and PLCγ1 phosphorylate PKCε on complementary sites to induce its activation .
We thank A. Ferrand and B. Delaval for discussions and L. Daviet (Hybrigenics, Paris) for two-hybrid screens results with CEP110. This work was supported by Inserm, Institut Paoli-Calmettes and grants from Institut National du Cancer (PL 2005 IdF-PACA).
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