Inhibition of HIF-1alpha activity by homeodomain-interacting protein kinase-2 correlates with sensitization of chemoresistant cells to undergo apoptosis
© Nardinocchi et al; licensee BioMed Central Ltd. 2009
Received: 04 November 2008
Accepted: 07 January 2009
Published: 07 January 2009
Homeodomain-interacting protein kinase-2 (HIPK2), a transcriptional co-repressor with apoptotic function, can affect hypoxia-inducible factor 1 (HIF-1) transcriptional activity, through downmodulation of its HIF-1α subunit, in normoxic condition. Under hypoxia, a condition often found in solid tumors, HIF-1α is activated to induce target genes involved in chemoresistance, inhibition of apoptosis and tumor progression. Here, we investigated whether the HIPK2 overexpression could downregulate HIF-1α expression and activity in tumor cells treated with hypoxia-mimicking condition, and evaluated whether HIPK2-dependent downregulation of HIF-1α could sensitize chemoresistant tumor cells to adriamycin (ADR)-induced apoptosis.
Tumor cell lines carrying wild-type p53, siRNA p53, or mutant p53 were overexpressed with HIPK2 (full length or catalytic inactive mutant) and treated with cobalt chloride (CoCl2) to mimic hypoxia, in the presence or absence of ADR treatment. HIF-1α expression was measured by semiquantitative reverse-transcriptase (RT)-PCR and Western immunoblotting and HIF-1 activity was evaluated by luciferase assay using reporter plasmid containing hypoxia response elements (HREs) upstream of luciferase gene. HIF-1 target genes, including multidrug resistance 1 (MDR1) and the antiapoptotic Bcl2 were determined by RT-PCR. Cell survival and apoptosis were measured by colony assay and cleavage of the caspase-3 substrate PARP, respectively.
Overexpression of HIPK2 resulted in downmodulation of cobalt-stabilized HIF-1α protein and HIF-1α mRNA levels, with subsequent inhibition of HIF-1 transcriptional activity. MDR1 and Bcl-2 gene expression was downmodulated by HIPK2 overexpression in cobalt-treated cells. Inhibition of HIF-1 transcriptional activity was dependent on HIPK2 catalytic activity. HIPK2 overexpression did not induce per se apoptosis of cobalt-treated cells, on the contrary it sensitized cobalt-treated cells to ADR-induced apoptosis, regardless of their p53 status.
The ability of HIPK2 to restore the apoptosis-inducing potential of chemotherapeutic drug in hypoxia-mimicking condition and therefore to sensitize chemoresistant tumor cells suggests that HIPK2 may induce fundamental alterations in cell signaling pathways, involving or not p53 function. Thus potential use of HIPK2 is promising for cancer treatment by potentiating cytotoxic therapies, regardless of p53 cell status.
Homeodomain-interacting protein kinase-2 (HIPK2) is a serine/threonine kinase  that as been shown to be involved in restraining tumor progression. HIPK2 carries out its potential oncosuppressor function through both its catalytic and transcriptional co-repressor activities. We and others have found that HIPK2 phosphorylates p53 at serine 46 (Ser46) after severe DNA damage and induces apoptosis [2, 3]; we also found that silencing of HIPK2 inhibits p53 transcriptional activity , inhibits drug-induced apoptosis inducing chemoresistance , and increases genomic instability , moreover, we showed that HIPK2 ovrexpression circumvents the blockade of apoptosis in cisplatin-resistant cells, restoring the p53-dependent apoptosis . The role of HIPK2 in regulating tumor growth has been also evidenced in p53-independent manner. We have shown that HIPK2 downregulates the cPLA2-induced prostaglandin E2 (PGE2) production, and that HIPK2 silencing increases in vivo tumor growth and tumor vascularity [8, 9]. Thus, we have found that HIPK2 can suppress the β-catenin-mediated transcriptional activation of vascular endothelial growth factor (VEGF) . Furthermore, we have recently found that HIPK2 binds to the hypoxia-inducible factor 1α (HIF-1α) promoter, in a co-repressor complex with histone deacetylase 1 (HDAC1), to repress HIF-1α at transcriptional level with subsequent suppression of HIF-1 transcriptional activity, in normoxic condition .
HIF-1 is a heterodimeric transcription factor that transactivates more than 60 target genes involved in multiple aspects of tumorigenesis including tumor growth, angiogenesis, metastasis, and chemotherapy response . HIF-1 consists of two subunits, HIF-1α and HIF-1β : HIF-1β is constitutively expressed in cells, while HIF-1α stability is stimulated by hypoxia, growth factors, and several oncogenes  thus, HIF-1α is overexpressed in many types of tumors and contributes to chemoresistance . Activation of HIF-1 depends primarily upon redox-sensitive stabilization of its α subunit  however, HIF-1 responsive genes can be induced by cobalt ions with kinetics similar to that of hypoxia induction .
The mechanisms of chemoresistance appear to be multifactorial and include, among others, inhibition of apoptosis and dysfunction of p53 oncosuppressor . Growing evidence suggests that hypoxia in tumors selects for cells with decreased potential for apoptosis through for instance the overexpression of the antiapoptotic gene Bcl-2 and decreased killing effect through upregulation of drug transporter proteins that may contribute to resistance to standard radio- and chemo-therapy [11, 18]. Thus, multidrug resistance (MDR) is a serious obstacle for cancer chemotherapy . The MDR phenotype is associated with induction of the MDR1 gene  which encodes for P-glicoprotein a transporter protein able to extrude a range of anti-cancer drugs out of the cell, lowering their concentration below the level required for cytotoxic effects . MDR1 has been shown to be HIF-1 target gene  and is involved in the acquisition of multidrug resistance in various types of cancers cells, for instance, tumors resistance to doxorubicin is associated with increased levels of MDR1 gene .
A recent survey of malignant and normal tissues found that the expression of HIF-1α is commonly increased in a variety of human tumors including colorectal carcinoma and glioblastoma . The purpose of our investigation was to evaluate, in colon cancer and glioblastoma cell lines, whether HIPK2 might inhibit HIF-1α expression and activity in hypoxia-mimicking condition and sensitize chemoresistant tumor cells to adriamycin (ADR)-induced apoptosis. The results show that overexpression of HIPK2 inhibited HIF-1α mRNA levels and cobalt-stabilized HIF-1α protein, with subsequent suppression of HIF-1 transcriptional activity. The HIPK2-mediated inhibition of HIF-1α correlated with suppression of MDR1 and Bcl-2 gene transcription and with sensitization of cobalt treated-tumor cells, regardless of their p53 status, to ADR-induced apoptosis. Thus, HIPK2 could be useful in cancer therapy to enhance the effectiveness of chemotherapeutic drugs.
HIPK2 downregulates HIF-1α expression and protein levels in hypoxia-mimicking condition
HIPK2 inhibited the HIF-1α transcriptional activity of target genes
Next we evaluated whether HIPK2 could suppress HIF-1 target gene transcription. To this aim, RKO-C, RKO-p53i, and T98G cells were transfected with HIPK2 or K221R and soon after transfection treated with CoCl2 for 24 h. Semiquantitative RT-PCR analysis of mRNA levels showed that the MDR1 and Bcl2 gene induction in cobalt treated-RKO-C cells was strongly suppressed by HIPK2 overexpression but not by K221R mutant (Fig. 2B). On the other hand, the already elevated MDR1 and Bcl2 mRNA levels in RKO p53i and in T98G cells, compared to RKO-C cells, were not further increased by cobalt treatment, at least at the time point analyzed; however, they were strongly suppressed by HIPK2 overexpression (Fig. 2B). Altogether, these results indicate that HIPK2 inhibited HIF-1 transcriptional activity, in cobalt-treated cells, suppressing MDR1 and Bcl2 gene expression.
Effect of HIPK2 on cell death and survival after cobalt treatment
HIPK2 sensitizes chemoresistant cells to ADR-induced apoptosis in hypoxia-mimicking condition
Our studies provide a rationale for the potential use of HIPK2 transduction to sensitize chemosensitive tumor cells to ADR-induced apoptosis. We show that HIPK2 downregulated HIF-1α levels in hypoxia-mimicking condition, suppressing HIF-1 transcriptional activity. Thus, HIF-1 target genes such as MDR1 and Bcl-2 were downmodulated by HIPK2 overexpression in cobalt-treated cells: downmodulation of HIF-1α, MDR1, and Bcl2 correlated with ADR-induced apoptosis. Thus, HIPK2 overexpression did not induce per se apoptosis of cobalt-treated cells, on the contrary it sensitized cobalt-treated cells to ADR-induced apoptosis, regardless of their p53 status.
We have previously documented the role of HIPK2 in drug-induced apoptosis. HIPK2 activation by chemotherapeutic drugs, including ADR and cisplatin, results in p53 phosphorylation at Ser46 with specific induction of apoptosis [4, 5]; on the other hand, HIPK2 is unable to induce apoptosis in p53 mutant cells , although p53-independent apoptotic pathways activated by HIPK2 have been reported [23–25]. We have recently shown that HIPK2 deregulation is involved in chemoresistance however, HIPK2 overexpression circumvents the blockade of apoptosis in chemoresistant cells, restoring activation of p53Ser46 and the caspases' pathway . Therefore, lack of functional p53 contributes to resistance to HIPK2 apoptotic function although, deregulation of the upstream signaling pathways and of the regulatory proteins that finally lead to HIPK2 activation in response to different stimuli cannot be excluded.
In the present study, we have shown that the exogenous HIPK2 might enhance the ADR-apoptotic response impaired by hypoxia-mimicking condition. It is noteworthy that HIPK2 sensitized to ADR-induced apoptosis also the RKO-p53i and T98G cells (carrying mutant p53) that showed an inherent ADR resistance in anoxic condition (Fig. 4A) and also resistance to HIPK2-induced long-term cell death (Fig. 3B). These data suggest that HIPK2 might induce fundamental alterations in cell signaling pathways, involving or not p53-dependent apoptotic function. In this regard, we have recently found that HIPK2 downmodulates HIF-1α expression in normoxic condition, repressing HIF-1 transcriptional activity . HIF-1α is a negative factor for tumor therapy that has a powerful role in modulating chemotherapeutic responses . The expression of multidrug resistance (MDR) via HIF-1-dependent regulation has been shown to increase chemoresistance . Our results showed that overexpression of HIPK2 downmodulated HIF-1α expression and HIF-1 transcriptional activity, in hypoxia-mimicking condition. Blocking of HIF-1α expression abolished MDR1 and Bcl2 expression and restored ADR-induced apoptosis. Reversal of MDR1 and Bcl2 upregulation could therefore provide the mechanistic basis for chemosensitization observed during HIPK2 transduction.
In summary, this study shows the efficacy of HIPK2 transduction to restore the apoptosis-inducing potential of chemotherapeutic drug in hypoxia-mimicking condition and therefore to sensitize chemoresistant tumor cells, regardless of their p53 status.
We present here a potential approach of combining HIPK2 transduction with chemotherapeutic drug for the treatment of chemoresistant tumor cells. Specifically, HIPK2 restored the apoptosis-inducing potential of chemotherapeutic drug in hypoxia-mimicking condition and sensitized chemoresistant tumor cells. These data suggest that HIPK2 might induce fundamental alterations in cell signaling pathways, involving or not p53 function and provide a foundation for the development of combined regimens that would enhance the apoptotic response of chemoresistant cells. Thus potential use of HIPK2, by either gene therapy deliver or identification of small peptides able to activate HIPK2, is promising for cancer treatment by potentiating cytotoxic therapies, regardless of p53 cell status.
Cell cultures and treatments
Human colon carcinoma RKO (carrying wtp53), RKO-p53-interfered (p53i) (a kind gift of Silvia Soddu, Regina Elena Cancer Institute, Rome Italy), the human glioblastoma T98G (mutant p53, codon 237 methionine-to-isoleucine change) , and human lung adenocarcinoma H1299 cells were cultured in RPMI-1640 (GIBCO-BRL, Life Technology, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (GIBCO-BRL) plus glutamine and antibiotics in humidified atmosphere with 5% CO2 at 37°C.
Adriamycin (ADR) was diluted into the medium to a final concentration of 2 μg/ml that we have shown can induce apoptosis in wild-type bearing cells but not in p53 inactive cells , for 16-24 h; hypoxia-mimicking condition was obtained by adding cobalt chloride (CoCl2) into the culture medium to a final concentration of 200 μM  for 24 h; cycloheximide (CHX), the inhibitor of protein synthesis, was diluted into the medium to a final concentration of 40 μg/ml for 3 h.
RNA extraction and semiquantitative reverse-transcriptase (RT)-PCR analysis
Cells were transfected with HIPK2-GFP, K221-GFP coding HIPK2 kinase defective mutant , and the empty GFP vector using LipofectaminePlus method. Soon after transfection cells were trypsinized, counted and equal cell number re-plated in 60 mm Petri dishes. Twelve hours after transfection cells were pre-treated with CoCl2 (200 μM) for 8 h and then treated with and ADR (2 μg/ml) for 16 h. Thirty-six hours after transfection, cells were harvested in TRIzol Reagent (Invitrogen) and total RNA was isolated following the manufacturer's instructions. The first strand cDNA was synthesized according to the manufacturer's instructions (Moloney murine leukemia virus reverse transcriptase kit, Applied). Semi-quantitative RT-PCR was carried out by using HOT-MASTER Taq (Eppendorf) with 2 μl cDNA reaction and genes specific oligonucleotides under conditions of linear amplification. The sequence of the primers used for RT-PCR was as follow: HIF-1α forward: 5'-CAGAAGATACAAGTAGCCTC-3'; HIF-1α reverse: 5'-CTGCTGGAATACTGTAACTG-3'; MDR1 forward: 5'-AACGGAAGCCAGAACATTCC-3'; MDR1 reverse: 5'-AGGCTTCCTGTGGCAAAGAG-3'; Bcl-2 forward: 5'-AGGA TTGTGGCCTTCTTTGAG-3'; Bcl-2 reverse: 5'-GAGACAGCCAGGAGAAATCAAA-3'. DNA products were run on 2% agarose gel and visualized by ethidium bromide using UV light. Densitometric analyses was applied to quantify mRNA levels. Data presented are representative of at least three independent experiments.
Viability assay and colony assay
Cells were transfected with HIPK2-GFP, K221-GFP and the empty GFP vector using LipofectaminePlus method (Invitrogen Corp., Carlsband, CA, USA). Soon after transfection cells were trypsinized, counted and equal cell number re-plated in triplicate in 60 mm Petri dishes. Twelve hours after transfection cells were pre-treated with CoCl2 (200 μM) for 8 h and then treated with and ADR (2 μg/ml) for 24 h. Both floating and adherent cells were collected and cell viability was determined by Trypan blue exclusion by direct counting with a haemocytometer, as reported .
For colony-formation assay, cells were transfected with expression vectors as above and soon after transfection treated with CoCl2 (200 μM) for 22 h before adding a pulse of ADR (2 μg/ml) for 2 h. After treatments, cells were washed, trypsinized, counted and equal cell number re-plated in duplicate with fresh medium, in 60 mm Petri dishes. Death-resistant colonies were stained with crystal violet 10-4 days later. Data presented are representative of at least three independent experiments.
Transfection, plasmids, and transactivation assay
Transient transfection was carried out using H1299 cells and the cationic polymer LipofectaminePlus method (Invitrogen Corp., Carlsband, CA, USA) according to manufacturers' instructions. The amount of plasmid DNA was equalized in each sample by supplementing with empty vector. Cells were transiently co-transfected with HIPK2-GFP or K221R-GFP expression vectors along with the luciferase reporter gene driven by human erythropoietin enhancer region containing a functional HIF-1 binding site (pEpoE-luc) or a mutant HIF-1 binding site (pEpoE-mut-luc)  (kindly provided by L. Eric Huang, N.C.I., N.I.H, Bethesda, MA, USA). Soon after transfection cells were trypsinized, counted and equal cell number re-plated in 60 mm Petri dishes. Twelve hours after transfection cells were pre-treated with CoCl2 (200 μM) for 8 h and then treated with and ADR (2 μg/ml) for 16 h. Transfection efficiency was normalized with the use of a co-transfected β-galactosidase (β-gal) plasmid. Thirty-six hours after transfection, cells were harvested and luciferase activity was assayed on whole cell extract. Luciferase values were normalized to β-galactosidase activity and protein content. At least three independent experiments were performed in duplicate.
Western blot analysis
Total cell extracts were prepared by incubating at 4°C for 30 min in lysis buffer (50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM EDTA, 150 mM KCl, 1 mM dithiothreitol, 1% Nonidet P-40) plus a mix of protease and phosphatase inhibitors (Sigma Chemical Company, MO, USA). Nuclear extracts were prepared essentially as described . Proteins were then separated by 9% SDS-PAGE and blotted onto nitrocellulose (BioRad, CA, USA). The membranes were probed with a primary antibody followed by with horseradish-peroxidase conjugated secondary antibody. The antibodies used were: monoclonal anti-HIF-1α (Novus Biologicals, UCS Diagnostic, Italy), monoclonal anti-poly(ADP-ribose) polymerase (PARP, BD Pharmingen, CA, USA), and monoclonal anti-Hsp70 (Stressgene, BC, Canada). Immunoreactivity was detected with the Advanced-ECL chemoluminescence reaction kit (Amersham., IL, USA).
Continuous variables were analyzed by the Student t test. Data are expressed as mean ± SD. A value of p ≤ 0.05 was considered statistically significant.
hypoxia response elements
This work was partly supported by Grants from Associazione Italiana per la Ricerca sul Cancro (AIRC). R.P. is a recipient of Fondazione Italiana per la Ricerca sul Cancro (FIRC) Fellowship. The authors wish to thank Dr. A. Aiello for helpful advice and stimulating discussion.
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