NC2213: a novel methionine aminopeptidase 2 inhibitor in human colon cancer HT29 cells
© Selvakumar et al; licensee BioMed Central Ltd. 2009
Received: 19 June 2009
Accepted: 24 August 2009
Published: 24 August 2009
Methionine aminopeptidase 2 (MetAP2) is a bifunctional protein that plays a critical role in the regulation of post-translational processing and protein synthesis. MetAP2 is overexpressed in human colon cancer. In this report we screened various MetAP2 inhibitors and treated HT29 cells with various concentrations of compounds. We evaluated the expression of MetAP2 and pp60c-src expressions in HT29 cells. In addition we also carried out the cell proliferation and cell cycle analysis in the MetAP2 inhibitor-treated HT29 cells. The cell cycle analysis of HT29 treated with 1.0 μM of NC2213 showed an arrest in the G2 phase followed by an induction in the percentage of cells undergoing apoptosis in the sub-G1 phase. Western blot analysis revealed that the MetAP2 expression was dose-dependently decreased when the HT29 cells were treated with the 3,5-bis(benzylidene)-4-piperidone derivative (NC2213). In addition, phosphorylation of Src, a myristoylated oncoprotein was significantly decreased by 1.0 μM of NC2213 as revealed by Western blot analysis. Furthermore, NC2213 also inhibits the expression of pp60c-src in HT29 cells. Interestingly, this compound also inhibits the phosphorylation at Tyr416 of pp60c-src while increasing the phosphorylation at Tyr527 of pp60c-src. NC2213 inhibits the growth of HT29 cells by inducing apoptosis and might be useful for the treatment of human colon cancer.
Methionine aminopeptidases (MetAPs) are the enzymes responsible for the removal of methionine from the amino-terminus of newly synthesized proteins [1, 2] which is essential for further amino terminal modifications (e.g., myristoylation of glycine by N-myristoyltransferase, NMT) [3, 4]. Various reports suggested that MetAP2 plays an important role in the growth of different types of tumors . Anti-sense of MetAP2 also induces apoptosis in rat hepatoma cells . A recent study suggested that fumagillin effectively inhibits both liver tumor growth and metastasis in rats in vivo . Higher MetAP2 expression was reported in malignant mesothelioma , malignant lymphomas  and esophageal squamous carcinomas . The angiogenesis inhibitor TNP470, O-(chloro-acetyl-carbamoyl) fumagillol, a synthetic analogue of fumagillin, suppressed the expression of MetAP2 in human neuroblastoma and thus, MetAP2 may be an important molecular target for human neuroblastomas . Earlier, we demonstrated the high expression of MetAP2 in colorectal adenocarcinoma patients . It appears that higher expression of MetAP2 is required for the overexpression of NMT in colon carcinogenesis.
c-Src is frequently observed to be activated or overexpressed in a number of human cancers, especially those of colon and breast. Activation of c-Src is achieved upon its myristoylation for proper signal transduction. Since myristoylation reaction is catalyzed by NMT, we reported that a cross-talk among the MetAP2, NMT, and N-myristoyltransferase inhibitor protein 71 (NIP71) in HT29 cells . Importantly, the Src family kinases have been shown to play pivotal roles in cell-cycle progression, making them potential candidates to mediate the cell-cycle effects of MetAP inhibitors. Western blot analysis revealed that phosphorylation of Src was significantly decreased by 1.0 μM of NC2213 (Figure 3B). We further investigated the molecular events associated with NC2213-induced cell cycle arrest by measuring the phosphorylation of Src, a myristoylated protein, is elevated in human colon cancer (Figure 3B). The phosphorylation of Src at Tyr417 and Tyr527 in HT29 cells was measured by Western blot analysis (Figure 3B). These observations lead us to the possibility of developing MetAP2 specific inhibitors, which may be therapeutically useful. In addition, it is important to study the regulation of MetAP2 and its involvement of various signal transduction pathways.
The possible role of MetAP2 and NMT in cell proliferation may be due to the interaction of these enzymes with various apoptotic factors. Increased expression of NMT in p53 mutant cases suggests that wild-type p53 may have a negative regulatory effect on NMT gene expression . MetAP2 plays a critical role in the proliferation of endothelial cells and certain tumor cells and thus serves as a promising target for anti-angiogenesis and anticancer drugs . It has been demonstrated that there is a high expression of MetAP2 in human mesothelioma tissue, and the association of this expression with anti-apoptotic function in those neoplastic cells . In addition, the inhibition of MetAP2 expression in mesothelioma cells leads to cell death and that such apoptosis is avoided in cases where there is overexpression of Bcl-2 . The upregulation of Bcl-2 in colorectal cancer is well established by various investigators [28–30]. One of the other mechanisms correlating with MetAP2 and apoptosis is through caspases . A recent observation suggested that mesalazine-induced apoptosis in colon cancer cells is possible through activation of caspase-3 . Chen et al reported a reduction in protein levels of caspase-3, caspase-7, and caspase-9 in human colon cancer specimens .
In summary, we have identified a novel MetAP2 inhibitor NC2213, which is a structurally divergent to fumagillin, in HT29 cells. The MetAP2 expression was dose-dependently decreased when the HT29 cells were treated with NC2213. NC2213 also inhibits the expression of pp60c-src and the phosphorylation at Tyr417 while increasing the phosphorylation at Tyr527 of pp60c-src in HT29 cells. Future detailed studies related to MetAP2 and apoptosis will shed light on the involvement of this enzyme in cell proliferation.
Materials and methods
Anti-MetAP2 was purchased from Zymed Laboratories Inc., (USA). Anti-pp60c-src, anti-phospho-Src (Tyr527], and anti-phospho-Src (Tyr416] were purchased from Cell Signaling, USA. Cell culture media, anti-β-Actin and other reagents were from Invitrogen and Sigma. NC2213 was synthesized as described earlier (Figure 1) .
Cell Culture and Cell Lysate Preparation
The HT29 cells were grown as described elsewhere .
Cell Proliferation Assay
Cells were dissociated with 2.5 g/L trypsin and resuspended in tissue culture media. Cells (1 × 105 cells/mL) were added to 96-well plates (9000 cells/well) for 24 h at 37°C. NC2213 was added at various concentrations ranging from 0-5 μM. After the cells were incubated with NC2213 for 96 h, cell proliferation was estimated based on the cellular reduction of tetrazolium salt MTT by using a micro plate reader (BIO-RAD, Model 550 USA) at 540 nm .
Cell Cycle Analysis
Cell cycle analysis was carried out as described elsewhere . Cells were treated with NC2213 at various concentrations ranging from 0-5 μM. After treatment for 48 h, cells were trypsinized, washed in PBS, and fixed overnight in 70% ethanol at 4°C. At the time of harvest, the cultures were 70-90% confluent. The ethanol solution was subsequently removed after centrifugation, and cells were resuspended in a buffer containing 10 mM Tris (pH 7.5), 125 mM sucrose, 2.5 mM MgCl2, 0.185% NP40, 0.02 mg/ml RNase A, 0.05% sodium citrate, and 25 μg/ml PI. After incubation on ice for 1 h, cells were subjected to DNA content analysis using a FACScan cytometer (Becton Dickinson).
SDS-Polyacrylamide Gel Electrophoresis and Western Blot Analysis
SDS-PAGE prepared according to the procedure described by Laemmli. Gel transfer to nitrocellulose membrane and blocking were performed using standard procedures . The blot was incubated with the primary antibodies at 1:1000, washed and probed with an anti-rabbit or anti-mouse IgG horseradish peroxidase conjugate diluted 1:2000.
Protein concentration was measured by the method of Bradford  using bovine serum albumin as a standard.
N-myristoyltransferase inhibitor protein 71
This work is supported by the Canadian Institutes of Health Research, Canada [RKS and JRD].
- Jackson R, Hunter T: Role of methionine in the initiation of haemoglobin synthesis. Nature. 1970, 227: 672-676. 10.1038/227672a0View ArticlePubMedGoogle Scholar
- Solbiati J, Chapman-Smith A, Miller JL, Miller CG, Cronan JE: Processing of the N termini of nascent polypeptide chains requires deformylation prior to methionine removal. J Mol Biol. 1999, 290: 607-614. 10.1006/jmbi.1999.2913View ArticlePubMedGoogle Scholar
- Selvakumar P, Lakshmikuttyamma A, Dimmock JR, Sharma RK: Methionine aminopeptidase 2 and cancer. Biochim Biophys Acta. 2006, 1765: 148-154.PubMedGoogle Scholar
- Selvakumar P, Lakshmikuttyamma A, Shrivastav A, Das SB, Dimmock JR, Sharma RK: Potential role of N-myristoyltransferase in cancer. Prog Lipid Res. 2007, 46: 1-36. 10.1016/j.plipres.2006.05.002View ArticlePubMedGoogle Scholar
- Datta R, Choudhury P, Ghosh A, Datta B: A glycosylation site, 60SGTS63, of p67 is required for its ability to regulate the phosphorylation and activity of eukaryotic initiation factor 2 alpha. Biochemistry. 2003, 42: 5453-5460. 10.1021/bi020699gView ArticlePubMedGoogle Scholar
- Datta B, Datta R: Induction of apoptosis due to lowering the level of eukaryotic initiation factor 2-associated protein, p67, from mammalian cells by antisense approach. Exp Cell Res. 1999, 246: 376-383. 10.1006/excr.1998.4313View ArticlePubMedGoogle Scholar
- Sheen IS, Jeng KS, Jeng WJ, Jeng CJ, Wang YC, Gu SL, Tseng SY, Chu CM, Lin CH, Chang KM: Fumagillin treatment of hepatocellular carcinoma in rats: an in vivo study of antiangiogenesis. World J Gastroenterol. 2005, 11: 771-777.PubMed CentralView ArticlePubMedGoogle Scholar
- Catalano A, Romano M, Robuffo I, Strizzi L, Procopio A: Methionine aminopeptidase-2 regulates human mesothelioma cell survival: role of Bcl-2 expression and telomerase activity. Am J Pathol. 2001, 159: 721-731.PubMed CentralView ArticlePubMedGoogle Scholar
- Kanno T, Endo H, Takeuchi K, Morishita Y, Fukayama M, Mori S: High expression of methionine aminopeptidase type 2 in germinal center B cells and their neoplastic counterparts. Lab Invest. 2002, 82: 893-901.View ArticlePubMedGoogle Scholar
- Bo H, Ghazizadeh M, Shimizu H, Kurihara Y, Egawa S, Moriyama Y, Tajiri T, Kawanami O: Effect of ionizing irradiation on human esophageal cancer cell lines by cDNA microarray gene expression analysis. J Nippon Med Sch. 2004, 71: 172-180. 10.1272/jnms.71.172View ArticlePubMedGoogle Scholar
- Morowitz MJ, Barr R, Wang Q, King R, Rhodin N, Pawel B, Zhao H, Erickson SA, Sheppard GS, Wang J, Maris JM, Shusterman S: Methionine aminopeptidase 2 inhibition is an effective treatment strategy for neuroblastoma in preclinical models. Clin Cancer Res. 2005, 11: 2680-2685. 10.1158/1078-0432.CCR-04-1917View ArticlePubMedGoogle Scholar
- Selvakumar P, Lakshmikuttyamma A, Kanthan R, Kanthan SC, Dimmock JR, Sharma RK: High expression of methionine aminopeptidase 2 in human colorectal adenocarcinomas. Clin Cancer Res. 2004, 10: 2771-2775. 10.1158/1078-0432.CCR-03-0218View ArticlePubMedGoogle Scholar
- Pati HN, Das U, Quail JW, Kawase M, Sakagami H, Dimmock JR: Cytotoxic 3, 5-bis(benzylidene)piperidin-4-ones and N-acyl analogs displaying selective toxicity for malignant cells. Eur J Med Chem. 2008, 43: 1-7. 10.1016/j.ejmech.2007.03.010PubMed CentralView ArticlePubMedGoogle Scholar
- Tucker LA, Zhang Q, Sheppard GS, Lou P, Jiang F, McKeegan E, Lesniewski R, Davidsen SK, Bell RL, Wang J: Ectopic expression of methionine aminopeptidase-2 causes cell transformation and stimulates proliferation. Oncogene. 2008, 27: 3967-3976. 10.1038/onc.2008.14View ArticlePubMedGoogle Scholar
- Abe J, Zhou W, Takuwa N, Taguchi J, Kurokawa K, Kumada M, Takuwa Y: A fumagillin derivative angiogenesis inhibitor, AGM-inhibits activation of cyclin-dependent kinases and phosphorylation of retinoblastoma gene product but not protein tyrosyl phosphorylation or protooncogene expression in vascular endothelial cells. Cancer Res. 1470, 54: 3407-3412.Google Scholar
- Adams BK, Ferstl EM, Davis MC, Herold M, Kurtkaya S, Camalier RF, Hollingshead MG, Kaur G, Sausville EA, Rickles FR, Snyder JP, Liotta DC, Shoji M: Synthesis and biological evaluation of novel curcumin analogs as anti-cancer and anti-angiogenesis agents. Bioorg Med Chem. 2004, 12: 3871-3883. 10.1016/j.bmc.2004.05.006View ArticlePubMedGoogle Scholar
- Hu X, Dang Y, Tenney K, Crews P, Tsai CW, Sixt KM, Cole PA, Liu JO: Regulation of c-Src nonreceptor tyrosine kinase activity by bengamide A through inhibition of methionine aminopeptidases. Chem Biol. 2007, 14: 764-774. 10.1016/j.chembiol.2007.05.010PubMed CentralView ArticlePubMedGoogle Scholar
- Dezube BJ, Von Roenn JH, Holden-Wiltse J, Cheung TW, Remick SC, Cooley TP, Moore J, Sommadossi JP, Shriver SL, Suckow CW, Gill PS: Fumagillin analog in the treatment of Kaposi's sarcoma: a phase I AIDS Clinical Trial Group study. J Clin Oncol. 1998, 16: 1444-1449.PubMedGoogle Scholar
- Kruger EA, Figg WD: TNP-470: an angiogenesis inhibitor in clinical development for cancer. Expert Opin Investig Drugs. 2000, 9: 1383-1396. 10.1517/135437188.8.131.523View ArticlePubMedGoogle Scholar
- Kudelka AP, Levy T, Verschraegen CF, Edwards CL, Piamsomboon S, Termrungruanglert W, Freedman RS, Kaplan AL, Kieback DG, Meyers CA, Jaeckle KA, Loyer E, Steger M, Mante R, Mavligit G, Killian A, Tang RA, Gutterman JU, Kavanagh JJ: A phase I study of TNP-470 administered to patients with advanced squamous cell cancer of the cervix. Clin Cancer Res. 1997, 3: 1501-1505.PubMedGoogle Scholar
- Griffith EC, Su Z, Niwayama S, Ramsay CA, Chang YH, Liu JO: Molecular recognition of angiogenesis inhibitors fumagillin and ovalicin by methionine aminopeptidase 2. Proc Natl Acad Sci USA. 1998, 95: 15183-15188. 10.1073/pnas.95.26.15183PubMed CentralView ArticlePubMedGoogle Scholar
- Antoine N, Greimers R, De Roanne C, Kusaka M, Heinen E, Simar LJ, Castronovo V: AGM - a potent angiogenesis inhibitor, prevents the entry of normal but not transformed endothelial cells into the G1 phase of the cell cycle. Cancer Res. 1470, 54: 2073-2076.Google Scholar
- Kusaka M, Sudo K, Matsutani E, Kozai Y, Marui S, Fujita T, Ingber D, Folkman J: Cytostatic inhibition of endothelial cell growth by the angiogenesis inhibitor TNP-470 [AGM-1470]. Br J Cancer. 1994, 69: 212-216.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang J, Lou P, Henkin J: Selective inhibition of endothelial cell proliferation by fumagillin is not due to differential expression of methionine aminopeptidases. J Cell Biochem. 2000, 77: 465-473. 10.1002/(SICI)1097-4644(20000601)77:3<465::AID-JCB11>3.0.CO;2-MView ArticlePubMedGoogle Scholar
- Yeh JR, Mohan R, Crews CM: The antiangiogenic agent TNP-470 requires p53 and p21CIP/WAF for endothelial cell growth arrest. Proc Natl Acad Sci USA. 2000, 97: 12782-12787. 10.1073/pnas.97.23.12782PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang Y, Griffith EC, Sage J, Jacks T, Liu JO: Cell cycle inhibition by the anti-angiogenic agent TNP-470 is mediated by p53 and p21WAF1/CIP1. Proc Natl Acad Sci USA. 2000, 97: 6427-6432. 10.1073/pnas.97.12.6427PubMed CentralView ArticlePubMedGoogle Scholar
- Selvakumar P, Lakshmikuttyamma A, Lawman Z, Bonham K, Dimmock JR, Sharma RK: Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29. Biochem Biophys Res Commun. 2004, 322: 1012-1017. 10.1016/j.bbrc.2004.08.021View ArticlePubMedGoogle Scholar
- Rajala RV, Radhi JM, Kakkar R, Datla RS, Sharma RK: Increased expression of N-myristoyltransferase in gallbladder carcinomas. Cancer. 2000, 88: 1992-1999. 10.1002/(SICI)1097-0142(20000501)88:9<1992::AID-CNCR3>3.0.CO;2-GView ArticlePubMedGoogle Scholar
- Yu HG, Yu LL, Yang Y, Luo HS, Yu JP, Meier JJ, Schrader H, Bastian A, Schmidt WE, Schmitz F: Increased expression of RelA/nuclear factor-kappa B protein correlates with colorectal tumorigenesis. Oncology. 2003, 65: 37-45. 10.1159/000071203View ArticlePubMedGoogle Scholar
- Valassiadou KE, Stefanaki K, Tzardi M, Datseris G, Georgoulias V, Melissas J, Tsiftsis DD, Delides G, Kanavaros P: Immunohistochemical expression of p53, bcl-2, mdm2 and waf1/p21 proteins in colorectal adenocarcinomas. Anticancer Res. 1997, 17: 2571-2576.PubMedGoogle Scholar
- Reinacher-Schick A, Schoeneck A, Graeven U, Schwarte-Waldhoff I, Schmiegel W: Mesalazine causes a mitotic arrest and induces caspase-dependent apoptosis in colon carcinoma cells. Carcinogenesis. 2003, 24: 443-451. 10.1093/carcin/24.3.443View ArticlePubMedGoogle Scholar
- Chen T, Yang I, Irby R, Shain KH, Wang HG, Quackenbush J, Coppola D, Cheng JQ, Yeatman TJ: Regulation of caspase expression and apoptosis by adenomatous polyposis coli. Cancer Res. 2003, 63: 4368-4374.PubMedGoogle Scholar
- Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB: Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987, 47: 936-942.PubMedGoogle Scholar
- Lakshmikuttyamma A, Pastural E, Takahashi N, Sawada K, Sheridan DP, DeCoteau JF, Geyer CR: Bcr-Abl induces autocrine IGF-1 signaling. Oncogene. 2008, 27: 3831-3844. 10.1038/onc.2008.8View ArticlePubMedGoogle Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. 10.1038/227680a0View ArticlePubMedGoogle Scholar
- Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 1979, 76: 4350-4354. 10.1073/pnas.76.9.4350PubMed CentralView ArticlePubMedGoogle Scholar
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.