Fig. 4

Onco-condensate remodels H3K27ac. a Representative image of immunofluorescence for H3K27ac in the BJ fibroblasts with lentiviral expression of SS18-EGFP or SS18-SSX1-EGFP. Scale bars, 5 μm. b Violin plot shows the pixel intensity distribution of immunofluorescence for H3K27ac in (a). Outside and inside groups indicate the distribution of random pixels’ fluorescence intensity of H3K27ac normalized by Z score from outside and inside of SS18 or SS18-SSX1 condensates, respectively. Two-sided Wilcoxon test adjusted for multiple comparisons. n = 30 pixels, from 3 nuclei. ****p < 0.0001. c Representative image of immunofluorescence for H3K27ac and H2AK119ub in the BJ fibroblasts with lentiviral expression of SS18-SSX1. Scale bars, 5 μm. d Heatmap shows the intensity changes of H3K27ac occupancy at SS18-SSX1 binding sites upon expression of the onco-fusion protein. e Jointly analysis of Hi-C, CUT&Tag of histone modification, SS18-SSX1 and cohesin-CTCF at three representative loci in BJ fibroblasts with the expression of SS18-SSX or condensate-deficient SS18(YA)-SSX as control. The green boxes indicate the newly formed long-range interaction. The blue and black dotted lines indicate the intrinsic chromatin domains. f Venn plot shows the intersection of cohesin component RAD21 binding sites in BJ cells with the overexpression of SS18-SSX1 and SS18(YA)-SSX as control. SS, SS18-SSX1. g Venn plot shows the intersection of cohesin component SMC3 binding sites in BJ cells with the overexpression of SS18-SSX1 and SS18(YA)-SSX as control. SS, SS18-SSX1. h Venn plot shows the intersection of CTCF binding sites in BJ cells with the overexpression of SS18-SSX1 and SS18(YA)-SSX as control. SS, SS18-SSX1. i H3K27ac histone modification sites were divided into three groups by Venn plot, i.e., control specific sites, SS18-SSX1 specific sites and the overlapped sites. j The colocalization analysis between the overlapped H3K27ac sites in (i) with cohesin component RAD21 binding sites in BJ cells with the overexpression of SS18-SSX1 and SS18(YA)-SSX as control, respectively. SS, SS18-SSX1. k The colocalization analysis between the specific H3K27ac sites in (i) with cohesin component RAD21 binding sites in BJ cells with the overexpression of SS18-SSX1 and SS18(YA)-SSX as control, respectively. SS, SS18-SSX1