The expression of miR-148b in tissues and cell lines. (A), MiR-148b was detected in 106 gastric cancer patients by qRT-PCR. Data were presented as log2 of fold change of gastric cancer relative to non-tumor adjacent tissues. The cases below the line (log2 = -1) revealed >50% reduction in the miR-148b level. (B), The relative level of miR-148b in gastric cancer cell lines (MGC-803, SGC-7901, BGC-823, AGS) relative to normal gastric epithelial cell line (GES-1). (C), H & E staining and detection of miR-148b by in situ hybridization in serial sections from gastric cancer tissue and its matched non-tumor adjacent tissue. Images are overlay images with brown color representing miR-148b expression. Data are presented as mean ± SD from at least three separate experiments. *, P < 0.05; **, P < 0.01.