Skip to main content
Figure 5 | Molecular Cancer

Figure 5

From: Bmi-1 promotes invasion and metastasis, and its elevated expression is correlated with an advanced stage of breast cancer

Figure 5

Suppression of endogenous Bmi-1 inhibits cellular motility, invasion and transformation. (A) Bmi-1 expression was confirmed by RT-PCR and immunoblotting. It was dramatically decreased by RNA interference in MDA-MB-435S cells. (B) The Bmi-1 knockdown did not alter the proliferation of MDA-MB-435S. (C) The mobility was measured by testing the rate of wound closure at 0, 8, 16 hours (200X). (D) The invasive properties induced by FBS were analyzed by using the Matrigel-coated Boyden chamber assay (400X, ***P < 0.001). (E) Anchorage-independent growth was measured in soft agar (200X, ***P < 0.001). (F) The acini formation of cells was tested in Matrigel culture (200X).

Back to article page