Stable silence of CypA reduces wound healing ability, and thus migration ability of M139 cells in vitro. To measure cell migration potential, wound healing assay was carried out in M139-shV and M139-shCypA cells using RPMI 1640 medium supplemented with 0%, 2%, and 5% FBS. The wound width measured at 24 hours for each experiment was normalized to that measured at initial wound (0 hour), generating the relative migration distance. (A) Representative images taken from the same field at 0 and 24 hour. (B) Average relative wound width (relative migration distance) was compared among groups. Bar graphs represent means ± SD; n = 4, ** denotes P < 0.05 versus the control.