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Figure 1 | Molecular Cancer

Figure 1

From: A fiber-modified adenoviral vector interacts with immunoevasion molecules of the B7 family at the surface of murine leukemia cells derived from dormant tumors

Figure 1

Correlation between the efficiency of Ad5FB4-mediated transduction of murine dormant leukemia cells and the time of tumor dormancy. (A, B), DA1-3b cells and the DA1-3b-derived cell lines DA1-3b/35, DA1-3b/d90 and DA1-3b/d365 were incubated with ß-gal-expressing Ad5 (open square) or Ad5FB4 vector (black square) at 104 vp/cell for 2 h at 37°C in serum-free medium. The ß-gal activity was evaluated by flow cytometry at 72 h pi, and expressed as (A) the percentage of ß-gal-positive cells, or (B) mean fluorescence intensity (MFI). In (B), the numbers on top of the bars represented the fold enhancement of ß-gal activity relative to the value in DA1-3b cells, which was attributed the 1-value. (C), DA1-3b/d365 cells were incubated with Ad5-ßgal (open square) or Ad5FB4-ßgal (black square) at 104 vp/cell for 2 h at 37°C, harvested at different times pi as indicated on the x-axis, and assayed for ß-gal expression. The ß-gal activity was expressed as MFI. Symbols: **, p < 0.01; ***, p < 0.001.

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Molecular Cancer

ISSN: 1476-4598