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Figure 2 | Molecular Cancer

Figure 2

From: A fiber-modified adenoviral vector interacts with immunoevasion molecules of the B7 family at the surface of murine leukemia cells derived from dormant tumors

Figure 2

Cell surface expression of mouse CAR (mCAR) and immunoglobulin Fc receptors in DA1-3b and DA1-3b-derived cell lines. (A, B), CAR expression. Cells first incubated with Fc receptor blocking antibody (rat anti-mouse CD16/CD32), or the corresponding control isotype, were reacted with goat polyclonal antibody against mCAR followed by FITC-labeled secondary antibody (donkey anti-goat), and analyzed by flow cytometry. Results are expressed as (A) the percentage of mCAR-positive cells, and (B) MFI. The open bars in panels A and B correspond to the isotype-matched control antibodies. (C), Fc receptors. Cells incubated with Fc receptor blocking antibody (dotted line), or the corresponding control isotype (solid line), as above, were reacted with Alexa fluor® 488-labeled goat anti-rat IgG. Results are presented as conventional flow cytometry patterns.

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Molecular Cancer

ISSN: 1476-4598