Figure 2
From: Tumor cell-selective apoptosis induction through targeting of KV10.1 via bifunctional TRAIL antibody
K V 10.1 expression analysis and apoptosis induction by scFv62-TRAIL. A) Kv10.1 mRNA levels determined by quantitative real-time PCR. B) Apoptosis analysis using Annexin assay and flow cytometry of different cell lines treated with 50 U/ml scFv62-TRAIL or medium for 20 h (n = 2). C) Immunoblot analysis using anti-caspase-3 antibody: 1) medium supernatant of CHO-K1, scFv62 preparation, 2) scFv62-TRAIL preparation, 3) scFv62-TRAIL dialyzed through a 100 kDa cut-off membrane.