Figure 1

Infiltrating cells in transgenic tissue. Formalin fixed, tissue sections were immunostained with : A-B: anti-von Willebrand factor revealing blood vessels (brown stain) shown at original magnification x10 (see additional file 2, figure S1 for details); C-F: anti-CD3 to reveal T-cells (brown stain), at x100 (C, D) and x400 (E, F) original magnification; G-J: astra blue staining to reveal mast cells (blue) at x10 original magnification (G, H) with indicated areas enlarged in I and J; K-N: anti-myeloperoxidase to reveal neutrophils and monocytes (brown staining) at either x100 (K, L) or x400 (M) original magnification, with a region of the intensely stained epidermis (boxed in L) enlarged in (N). LMP1^CAO.117 transgenic ear phenotypic stage 2 and 5 (St2 and St5 respectively) and NSC (C2 and C5) samples were used. The epidermis, e, dermis, d, and cartilage, c, are indicated. Dashed lines indicate the epidermal basement membrane.