Figure 5

STAT3 is activated in the transgenic tissues and inflammatory factors induced. Protein was extracted from control (NSC: C2, C5) and transgenic ears (St2, St5). Three biological replicates (100 μg) were separated by SDS-PAGE and western blotted. A: 10% gel probed with α-CD153 (migrates at ~44kD); B: 10% gel probed with anti-CD30 (migrates at 120kD), note the non-specific bands in two C2 samples due to relatively high loading, with no CD30 specific band; C: 15% gel probed with anti-CXCL10 (migrates at 10kD); D: 15% gel probed with anti-IL-3 (migrates at 15kD); E: 10% gel probed sequentially with anti-phospho-ser727-STAT3 (migrates at 89kD) then anti-total-STAT3 (α isoform migrates at 86kD, β isoform migrates at 79kD). Detection of GAPDH was used as a loading control (37kD) shown below each panel.