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Figure 1 | Molecular Cancer

Figure 1

From: c-Myc dependent expression of pro-apoptotic Bim renders HER2-overexpressing breast cancer cells dependent on anti-apoptotic Mcl-1

Figure 1

RNA interference mediated knock-down of Mcl-1 induces cell death in HER2 overexpressing cells. BT474 cells were left untreated or transfected with control siRNA, Bcl-xL siRNA, Bcl-2 siRNA or Mcl-1 siRNA as indicated. (A) Down regulation of the targeted proteins 48 hours after transfection was confirmed by western blot analysis, using tubulin as a loading control. (B) Forty eight hours after transfection, apoptosis was assessed by Apo 2.7 staining and FACS analysis as described in Materials and Methods. Data represented (% of Apo2.7 positive cells) are the means +/- standard errors of three independent experiments. p-values were assessed using a Student's t test. (C) Experiments were performed as described in B, using HER2 overexpressing SKBR3 cells, and ER expressing MCF7 cells, as indicated. Data are mean ± se of the indicated number of independent experiments. p-values were assessed using a Student's t test

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