c-Myc dependent expression of pro-apoptotic Bim renders HER2-overexpressing breast cancer cells dependent on anti-apoptotic Mcl-1

Table 1 Impact of HER2 status on the expression of Bcl-2 family members in mammary tumors

Probe	Gene	pValue	Enrichment in HER2+
P_1861_at	BAD	0,0294	-
P_203728_at	BAK1	ns	ns
P_208478_s_at	BAX	ns	ns
P_211833_s_at	BAX	ns	ns
P_211692_s_at	BBC3	ns	ns
P_203685_at	BCL2	< 0.0001	-
P_207004_at	BCL2	0,0157	-
P_207005_s_at	BCL2	0,0311	-
P_203684_s_at	BCL2	ns	ns
P_205681_at	BCL2A1	ns	ns
P_212312_at	BCL2L1	0.0054	+
P_206665_s_at	BCL2L1	ns	ns
P_215037_s_at	BCL2L1	ns	ns
P_221320_at	BCL2L10	ns	ns
P_222343_at	BCL2L11	0.0061	+
P_208536_s_at	BCL2L11	ns	ns
P_217955_at	BCL2L13	ns	ns
P_221241_s_at	BCL2L14	ns	ns
P_209311_at	BCL2L2	ns	ns
P_211725_s_at	BID	0,0086	+
P_204493_at	BID	ns	ns
P_205780_at	BIK	0.016	ns
P_221454_at	BOK	ns	ns
P_206864_s_at	HRK	ns	ns
P_206865_at	HRK	ns	ns
P_214056_at	MCL1	0,0097	+
P_200796_s_at	MCL1	ns	ns
P_200797_s_at	MCL1	ns	ns
P_200798_x_at	MCL1	ns	ns
P_214057_at	MCL1	ns	ns
P_204285_s_at	PMAIP1	0,0005	-
P_204286_s_at	PMAIP1	< 0.0001	-

The impact of HER2 status (evaluated by immunohistochemistry) on the expression of 20 genes of the Bcl-2 family was evaluated as described in Methods. The cohort contained 41 HER2+ and 170 HER2- ones. + and - indicate an enrichment and an impoverishment, respectively, in the HER2 overexpressing cohort. P values were assessed by a Wilcoxon test. Pro-apoptotic genes are shown in bold.

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