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Figure 1 | Molecular Cancer

Figure 1

From: Oncogenic functions of hMDMX in in vitro transformation of primary human fibroblasts and embryonic retinoblasts

Figure 1

Generation of panels of transformed human skin fibroblasts and human embryonic retinoblasts. A. Schematic representation of transformation process. Primary human fibroblasts (VH10) were immortalized with human Telomerase (hTERT). In subsequent rounds of retroviral infection using the indicated constructs, followed by selection, several stable cell lines were created. B. Total cell extracts from all transformed fibroblast cell lines were analyzed by immunoblotting with the indicated antibodies. C. Human Embryonic Retinoblasts (HER) were similarly transformed according to the scheme. D. Total cell extracts from all transformed HER cell lines were analyzed by immunoblotting with the indicated antibodies.

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Molecular Cancer

ISSN: 1476-4598