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Table 1 mRNA expression levels in VH10 and HER cell lines

From: Oncogenic functions of hMDMX in in vitro transformation of primary human fibroblasts and embryonic retinoblasts

VH10

p21

hMDM2-p2

PUMA

VH10 wt

1.00

±

0.16

1.00

±

0.17

1.00

±

0.23

shp53

0.24

±

0.03

0.20

±

0.03

0.49

±

0.11

HRasV12-shp53

0.15

±

0.03

0.32

±

0.07

0.44

±

0.15

shRB-HRasV12-shp53

0.63

±

0.11

0.41

±

0.15

1.54

±

0.32

HA-hMDMX

0.47

±

0.09

0.61

±

0.08

0.41

±

0.08

HRasV12-HA-hMDMX

0.76

±

0.12

1.05

±

0.17

0.53

±

0.14

shRB-HRasV12-HA-hMDMX

1.05

±

0.12

1.14

±

0.15

0.28

±

0.07

HER

p21

hMDM2-p2

PUMA

HER wt

1.00

±

0.16

1.00

±

0.15

1.00

±

0.19

shp53

0.11

±

0.03

0.01

±

0.01

0.46

±

0.13

shRB-shp53

0.23

±

0.05

0.02

±

0.00

0.36

±

0.06

shRB-HRasV12-shp53

0.05

±

0.02

0.01

±

0.00

0.15

±

0.04

HA-hMDMX

1.51

±

0.26

1.43

±

0.28

0.67

±

0.30

shRB-HA-hMDMX

1.01

±

0.16

0.62

±

0.13

0.34

±

0.11

shRB-HRasV12-HA-hMDMX

2.52

±

0.70

0.61

±

0.17

0.60

±

0.17

  1. Total RNA of each cell line was isolated and expression levels of the indicated genes were determined by qRT-PCR and normalized for the housekeeping genes CAPNS1 and TBP; levels are shown relative to wild-type cells.