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Table 2 Protein levels, relative to untreated VH10 wt cells, corrected for HAUSP expression

From: Oncogenic functions of hMDMX in in vitro transformation of primary human fibroblasts and embryonic retinoblasts

Cell line

Nutlin-3 treatment (h)

p53

p21

hMDM2

 

0

1.00

1.00

1.00

VH10 wt

6

4.96

6.68

82.32

 

24

7.82

11.16

149.60

 

0

0.35

0.20

0.27

shp53

6

0.87

0.65

0.30

 

24

1.25

2.26

4.54

 

0

0.28

0.03

0.33

HRasV12-shp53

6

0.30

0.09

0.78

 

24

0.44

0.33

0.74

 

0

0.45

1.01

2.51

shRB-HRasV12- shp53

6

0.49

1.24

4.55

 

24

0.74

2.85

9.17

 

0

6.99

0.56

0.87

HA-hMDMX

6

9.72

2.86

47.81

 

24

11.70

2.10

17.19

 

0

1.63

0.63

0.69

HRasV12-HA-hMDMX

6

4.13

2.88

36.75

 

24

5.01

3.07

29.42

 

0

1.07

1.45

2.79

shRB-HRasV12-HA-hMDMX

6

2.38

5.37

132.80

 

24

1.76

4.28

79.91

  1. Band intensities shown in figure 3B were quantified using the Odyssey 2.1 analysis software (LI-COR Biosciences) and the relative protein levels were calculated using HAUSP expression as an internal control. Basal protein levels in VH10 wild-type cells were set at 1.0.
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Molecular Cancer

ISSN: 1476-4598