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Figure 3 | Molecular Cancer

Figure 3

From: Side population rather than CD133+ cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells

Figure 3

Lack of enhanced tumorigenicity of CD133+ enriched cells. A). Double FACS sorted CD133+ subpopulations were assayed for colony-forming advantage relative to the CD133- subpopulation. A) 2D colony-forming assay or B) sphere-forming assay. Data are expressed as means ± standard deviations. Statistical comparisons are with the CD133+ subpopulation, *P = 0.1; **P < 0.05. C). Schematic depiction of assay in mice with CD133-fractioned RC-58T/hTERT/SA#4-D cells as a representative of all tested hTERT-immortalized primary prostate cancer cell lines. In NOD-SCID mice, CD133- cells generated larger tumors than CD133+ cells. D). Formalin-fixed, paraffin-embedded tissues were obtained from xenograft tumors. Individual tissues sections were stained with H&E or human specific primary PSCA antibody, and Alexa Fluor-594 secondary (red) and Dapi (blue) nuclear stain. Scale bar indicates 40 μM.

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