Immunohistochemical detection of Dlx-2 in human tumors, including breast, colon, and ovarian cancers. IHC was performed on 4-μm sections of formalin-fixed, paraffin-embedded human tumors, including breast, colon, and ovarian tumor tissues. Sections were incubated with an anti-Dlx-2 antibody and the antibody was visualized with diaminobenzidine chromogen, and sections were counterstained with hematoxylin. Dlx-2, brown staining; nuclei, blue staining (H & E). A-C, breast cancer; D-F, colon cancer; G-I, ovarian cancer, and J, the enlargement image of panel I. Arrows in panel J indicate strong positive Dlx-2 staining in tumor cells adjacent to areas of necrosis and in the cells in the necrotic core. Scale bar, 200 μm.