Reduction of E-cad by siRNA increased EGFR mRNA in SCCHN cell lines. Both E-cad siRNA and non-targeted control siRNA were transfected using HiPerFect transfection reagent. The EGFR mRNA level was evaluated 24 h after the transfection by RT-PCR. RT-PCR on GAPDH was performed at the same time as a control. Three out of four cell lines (Tu686, 686LN, PCI-37A) showed increased expression of EGFR mRNA.