BRAFV600E induces loss of epithelial characteristics of colon adenocarcinoma cells. (A) Confocal images after phalloidin staining show the altered morphology of Caco-BR13 cells and filopodia formation in Caco-K cells (arrow). Representative confocal images are shown in high (i) and low (ii) cell density. (B) Light microscope and confocal images of phalloidin staining in 3D cultures illustrates the architecture of Caco-2 and derived oncogenic models after 12 days of growth in Matrigel. Caco-2 and Caco-K15 cells formed spheroids with the typical lumen (arrows), a structure completely absent in Caco-BR13 and Caco-H2 cells.