BRAFV600E impairs distribution of E-cadherin. (A) Real-Time PCR (upper panel) and western blot analysis of E-cadherin (lower panel). (B) Confocal images in 2D (upper panel) and 3D (lower panel) conditions indicate disorganized of E-cadherin localization and its significant reduction in Caco-BR13 cells. (C) Real-Time PCR (upper panel) and western blot analysis of Vimentin (lower panel). (D) Confocal images of Vimentin show no alteration of this protein in Caco-BR13 and Caco-2 cells. (E) Real-Time PCR (upper panel) and western blot analysis (lower panel) for N-cadherin indicates augmentation of this mesenchymal marker in Caco-BR cells. (F) Confocal images of N-cadherin. Indicated antibodies in the western blot analysis were hybridized on the same membrane and slower migrating band appearing above tubulin represents Vimentin. Real-Time PCR expression experiments were performed twice in triplicates and representative confocal images are shown.