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Figure 5 | Molecular Cancer

Figure 5

From: BRAF and RAS oncogenes regulate Rho GTPase pathways to mediate migration and invasion properties in human colon cancer cells: a comparative study

Figure 5

The impact of depleting RhoA in cell migration and invasion and its regulation by signalling pathways. (A) Caco-2 and Caco-BR cells treated for 48 hours with 80 pmol of a negative siRNA control sequence (siCtr) or with siRNA against RhoA and analysed by western blot with indicated antibodies. (B) Cell migration and cell invasion of Caco-2 and Caco-BR13 following siRNA of RhoA. (C) DLD-1 and DKO4 cells treated with 80 pmol of siRNA against RhoA for 48 hours and analyzed by western blot for RhoA. (upper panel). Cell migration ability was assayed 48-hour after treatment with siRNA against RhoA (lower panel). (D) HTps, HTshBR3 cells treated with 80 pmol of siRNA against RhoA for 48 hours and analyzed by western blot for RhoA (upper panel). Cell migration ability was assayed 48-hour after treatment with siRNA against RhoA (lower panel). (E) GST pull-down RhoA activation assay following treatment for 30 minutes with 10 μM of UO126 was performed in Caco-2 and Caco-BR13 cells (upper panel). Levels of p-cofilin and p-Myl were analysed by western blot following treatment with 1 μM wortmanin for 30-120 minutes (lower panel). *: P ≤ 0,05; **: P ≤ 0,01; ***: P ≤ 0,001 as determined with Student t-test.

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Molecular Cancer

ISSN: 1476-4598