Rac1 is important for cell migration and invasion properties of Caco-H cells. (A) GST-pull down assay for Rac1 activation in a panel of cell lines (left panel) and quantification (right panel). (B) Caco-2 and Caco-H2 cells were treated with 1 μM PI3K inhibitor wortmanin for 30 minutes and GST pull-down assay for Rac1 activity (upper panel) and RhoA activity (lower panel) was performed. (C) Caco-H2 cells were treated with Rac1 specific inhibitor NSC23766 for 36 hours at indicated concentrations after which a GST-pull down assay for Rac1 activation was performed. (D) Cell migration and invasion ability of Caco-H2 cell in the presence of Rac1 inhibitor NSC23766 was estimated after 36 hours (upper panel) and quantification.(lower panel) *: P ≤ 0,05 as determined with Student t-test.