Figure 5

Pharmacological properties of GMME1 on CCR2⁺ medulloblastoma cells. A. Confirmation of CCR2 expression on mouse medulloblastoma cell line PS125. B. PS125 cells were seeded in 6-well plates (10⁴ cells/well) and cultured with CCL2^-/- or GMME1 CM. After 48 hrs, apoptosis was measured by Annexin-V/PI staining. C. To further see a dose-response effect, the same experiment was repeated using 3 different concentrations and cell death was analyzed (P < 0.05; n = 3/group). D. Human medulloblastoma cells (Daoy) were analyzed by WB to confirm the presence of CCR2 on cell surface. E. Human medulloblastoma cells were treated with GMME1 as described in B. Cell apoptosis was measured by Annexin-V/PI staining after 48 hrs culture. F. Human medulloblastoma cells were cultured in presence of GMME1 in condition medium or affinity-purified GMME1 protein. Cell growth was measured by MTT assay. (*P < 0.05; **P < 0.01; n = 3/group).