GMME1 induced apoptosis of primary myeloma cells from patients. A. White blood cells were isolated from bone marrow aspirates from patients with myeloma, and stained with anti-human CD38, CD45 and CD138 antibodies. CD38+CD45-CD138+ cells were considered as myeloma cells. B-C. CCR2 highly expressed on myeloma cells (black field is antibody isotype control). D-E. Isolated lymphocytes were cultured in absence (D) or presence (E) of GMME1, and the apoptotic myeloma cells were determined by annexin staining (D and E). F. Percentage of apoptotic myeloma cells from patients was presented as the mean+/-SD (*P < 0.05; n = 5).