EFEMP1 suppression of tumorigenicity, in vivo cell proliferation, and VEGFA-induced angiogenesis. A, weights of s.c. xenografts dissected 24 days after implantation with 5 × 106 cells of U251HF and its EFEMP1 transfectants. B, total (THb) hemoglobin concentration and tissue oxygen saturation (StO2) of s.c. xenografts in live mice by modulated imaging before tumor dissections. C-D, immunofluorescence of tumor frozen-sections, with CD31 antibody detecting blood vessel density and Ki67 antibody detecting proliferation index after DAPI-counterstaining of the nuclei. E, real-time qRT-PCR quantification of gene expressions in s.c. tumors above, with mean and SD for 6-9 tumors, normalized to ACTB. F, tumor growth curve based on tumor volume measurement after s.c. implantation.