Figure 3
EFEMP1 knock-down promoted tumorigenicity and VEGFA production by two glioma primary cultures representing tumor mass cell populations. A, growth curves of s.c. xenograft from implantation of 1 × 106 cells of adherent primary cultures of GBM (GBM16B) and anaplastic oligodendroglioma (OG2B), infected with lentivirus of empty vector or shRNA of EFEMP1 (shEFEMP1). B, weights of above tumors dissected 28 days after implantation. C, immunoblotting of protein extracts from above s.c. xenografts with EFEMP1 and Actin antibodies (upper panel), and quantification of VEGFA protein level in tumor by an VEGF-165 immunometric assay in duplicates (bottom panel).