Effects of Docetaxel on apoptosis and proliferation in the PC-3, DU-145 and 22RV1 Docetaxel resistant sublines. A. The PC-3 Ag, PC-3 D8, PC-3 D12, DU-145 Ag, DU-145 R, 22RV1 Ag and 22RV1 R cell lines were treated with Docetaxel (20 and 80 nM) for 48 hrs. Apoptosis was assessed by propidium iodide DNA staining and flow cytometry. Values are expressed as mean ± standard deviation. An independent samples t-test was used to compare the mean percentage apoptosis for control, 20 nM and 80 nM for each cell line. **p < 0.001. B. Cell proliferation was measured using the MTT Assay. The PC-3 sublines (PC-3 Ag, PC-3 D8 and PC-3 D12) were treated with increasing doses of Docetaxel (10, 20, 50 & 100 nM) for 24, 48 and 72 hrs. Data shown is representative of three independent experiments.