Figure 5
From: Characterisation and manipulation of docetaxel resistant prostate cancer cell lines
Validation of Low Density Arrays. Total cellular protein was extracted from the PC-3 sublines (PC-3 Ag, PC-3 D8 & PC-3 D12) and 50 μg was loaded onto a western blot to assess the expression of Clusterin, HSP90, Id-1, Bcl-2, Bcl-xL, Bax, and Bid using their respective antibodies as described in the Method section. The house keeping protein β-actin was used as a loading control. Blots shown are representative of three independent experiments.