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Figure 2 | Molecular Cancer

Figure 2

From: Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation

Figure 2

UCHL1 expression is regulated by promoter methylation in PCa. (A) MSP results show highly (44, 45, 48 and 51) and weakly (49) methylated tumour samples as well as positive (M) and negative (U) controls. The M lane shows amplification with primers specific for methylated CpG sites and the U lane with primers specific for unmethylated CpG sites. The positive control is obtained from in vitro methylated DNA and the negative control from peripheral blood of a healthy donor. (B) The pyrogramm of one sample covers 12 CpG sites (grey bars) in the promoter region of UCHL1 with a methylation rate between 35 and 55% (mean 48%). Nucleotides confirming complete bisulfite conversion are shown in yellow bars. (C) The pyrosequencing of 20 normal and 20 prostate cancer samples revealed a significant higher rate of methylation for the cancer tissue. 18 tumour samples show a methylation density in the promoter region of UCHL1 clearly higher than the normal prostate tissue.

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Molecular Cancer

ISSN: 1476-4598