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Figure 4 | Molecular Cancer

Figure 4

From: Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation

Figure 4

Influence of UCHL1 overexpression on the phenotype of LNCaP cells. (A) Cell proliferation after overexpression of UCHL1 and cell viability was measured by using Vi-CELL Cell Viability Analyzer. (B) Colony formation assay in soft agar for LNCaP cells with (UCHL1) or without UCHL1 (Puro) expression. UCHL1 positive cells (top) show significant suppression in the number of colonies appearing in soft agar and in the colony morphology compared to mock cells (bottom). (C) After crystal violet staining the number of colonies appearing on soft agar plates was counted using an invert microscope and the results are expressed as mean ± SD of 3 independent experiments (p < 0.005). (D) Senescence was quantified using SA-ß-gal-staining. (E) Number of SA-ß-gal positive cells was counted using an Axioplan microscope and the results are depicted as mean ± SD of 3 independent experiments.

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