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Figure 5 | Molecular Cancer

Figure 5

From: Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation

Figure 5

In LNCaP cells exogenous expression of UCHL1 influences K63 ubiquitylation. (A) Representative Western blots against mono- and polyubiquitin from LNCaP cells stably expressing UCHL1 or empty vector as control. GAPDH is used as an internal housekeeping protein to ensure equal loading of samples. (B) Measurement of chymotrypsin like activity of the proteasome in UCHL1 positive or mock LNCaP cells served as control. The results are expressed as the mean ± SD from 3 independent experiments each in triplicates. (C) UCHL1 overexpression in LNCaP cells reduces the level of K63 chain specific ubiquitylated proteins detected by Western blotting with anti K63 linked polyubiquitin antibody. GAPDH is used as loading control.

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Molecular Cancer

ISSN: 1476-4598