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Figure 6 | Molecular Cancer

Figure 6

From: Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation

Figure 6

UCHL1 suppresses anchorage-independent growth and cell proliferation via AKT phosphorylation and stabilization of p53 levels. (A) Representative Western blots showing decreased levels of phospho AKT in UCHL1 positive LNCaP cells. Furthermore, accumulation of p53, decreased MDM2 levels and an increase in p14ARF without any influence on p21 was shown by Western blot. β-Actin was used as controls to ensure equal loading of samples. (B-C) Quantitative RT-PCR revealed no changes of p53 and MDM2 mRNA levels in UCHL1 positive cells. The results are indicated as mean ± SD of 3 independent experiments.

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Molecular Cancer

ISSN: 1476-4598