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Figure 7 | Molecular Cancer

Figure 7

From: Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation

Figure 7

Effects of overexpression of UCHL1 on the expression of cell cycle related proteins. (A) Representative Western blots against UCHL1, p27Kip1, Cyclin A and β-actin, as a house keeping protein, demonstrated that UCHL1 induces accumulation of p27Kip1 and reduction of Cyclin A expression in LNCaP cells. (B-C) Quantitative RTPCR revealed no statistically significant change of p27Kip1 and Cyclin A mRNA level in UCHL1 positive cells. The results are indicated as mean ± SD of 3 independent experiments. (D) Protein levels of Rb, pRb, Cyclin E, Cyclin D and β-actin were detected by Western blotting analyses using corresponding antibodies in UCHL1 overexpressing LNCaP cells. (E) Quantitative RTPCR revealed no statistically significant change of Cyclin D mRNA level in UCHL1 positive cells. The results are indicated as mean ± SD of 3 independent experiments.

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Molecular Cancer

ISSN: 1476-4598