Time course of phospho-STAT1 and STAT1 protein levels in IFNγ-stimulated DSL-6A/C1 cells. The cells were stimulated under serum-free conditions with IFNγ (100 ng/ml) for up to 720 min. Total cellular lysates from an equal number of cells were subjected to immunoblot analysis. Primary antibodies were detected with the help of fluorescein (IRDye®)-labeled secondary antibodies. Phospho-STAT1 (STAT1P), STAT1 protein and ERK 1/2 staining are exemplarily shown for one blot. The samples were loaded in a randomized order to exclude lane correlated blotting errors caused by gel and transfer inhomogeneities, as previously suggested .