Effects of IFNγ at 10 ng/ml on STAT1 protein expression and phosphorylation. Experimental data: DSL-6A/C1 cells were stimulated with IFNγ for the indicated periods of time. Phospho-STAT1 and STAT1 protein levels in total cellular lysates were determined by immunoblot analysis. The data were processed as described in the legend to Fig. 5. Averaged values (± SEM) of four independent experiments are presented by blue symbols. Computational simulations: The simulated time courses resulting from the mathematical model are presented by solid red lines.