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Figure 5 | Molecular Cancer

Figure 5

From: Specific knockdown of uPA/uPAR attenuates invasion in glioblastoma cells and xenografts by inhibition of cleavage and trafficking of Notch -1 receptor

Figure 5

puPA, puPAR and pU2 inhibits trafficking of Notch 1 receptor in U251 MG cells. (A) U251 MG cells left untreated (panels a to c) or transfected with pSV (panels d to f), pupa (panels g to i), puPAR (panels j to l) and pU2 (panels m to o) were washed, fixed and permeabilized, and then blocked with normal goat serum and incubated with primary antibodies against Notch 1 and LAMP-1 for 1 hr at RT. The cells were then washed and incubated with Alexa fluor 488 and Alexa fluor 594 conjugated appropriate secondary antibodies. The cells were then washed, and mounted with an anti-fade reagent and viewed under an Olympus fluoview fluorescent microscope. Arrows indicate areas of co-localization. (B) Quantitative representation showing percentage cell count with co-localization of Notch 1 and LAMP1. Data represents average of three independent experiments (**P < 0.01, *P < 0.05; in comparison with the controls).

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Molecular Cancer

ISSN: 1476-4598