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Figure 6 | Molecular Cancer

Figure 6

From: Specific knockdown of uPA/uPAR attenuates invasion in glioblastoma cells and xenografts by inhibition of cleavage and trafficking of Notch -1 receptor

Figure 6

puPA, puPAR and pU2 inhibits Notch 1 expression in pre established intracranial tumors in nude mice. (A) U251 MG cells in suspension (2 × 106 in 10 μl serum-free medium) were injected by the intracranial route. One week later, the mice were injected with either pSV or shRNA-expressing vectors (puPA and pU2) using an Alzet mini osmotic pump (constructs diluted to 1.5 μg ml-1 in PBS and injected at 0.25 μg hour-1, with six mice in each group). After a 5-week follow-up period, mice were sacrificed, their brains removed, paraffin embedded and sectioned. Sections were observed and immunohistochemical analysis for Notch 1 was done on paraffin-embedded brain tumor sections of mice treated with pSV, puPA and pU2 with specific antibody for Notch 1.

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Molecular Cancer

ISSN: 1476-4598