Figure 6
Spindle pole fragmentation in Aurora-Ai mitoses depends on active Nuf2. A. The efficiency of Aurora-A and Nuf2 depletion after RNAi was assessed by WB (left panels) and IF (right panels) analyses. B. Histograms represent the percentage of PM/M displaying fragmented spindle poles (alpha-tubulin staining) after transfection with the indicated siRNAs (at least 200 cells per condition, 2 experiments). C. Elongated spindles induced by Nuf2 RNAi are not rescued in Aur-Ai/Nuf2i co-inactivated cells (about 200 counted PM/M per condition in 2 experiments). Spindle axes are schematised on the right: the pole-to-pole axis is longer in Nuf2i cells compared to controls. Error bars denote s.d. *: p < 0.01, **: p < 0.001, χ2 test. Scale bar: 10μm.