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Figure 2 | Molecular Cancer

Figure 2

From: HCCS1-armed, quadruple-regulated oncolytic adenovirus specific for liver cancer as a cancer targeting gene-viro-therapy strategy

Figure 2

Ad.SPDD shows less toxicity and an enhanced efficacy at lysing cancer cells compared to ZD55. (A) Specific expression of E1A in Huh-7 cells infected with Ad.SPDD. MRC-5 and Huh-7 cells were infected with Ad.SPDD, ZD55 and Ad.WT or without any infection, and the expression of E1A was detected 48 hours post infection, with actin used as loading control. (B) The replicative ability of the adenoviruses in MRC-5 and Huh-7 cells. MRC-5 and Huh-7 cells were infected at an MOI of 10, and the adenovirus titer was measured 48 hours after infection. The results are presented as the mean+SD (n = 3). (C) The adenovirus titer 8 hours after adenovirus infection of MRC-5 and Huh-7 cells. The adenovirus titers in the MRC-5 and Huh-7 cells were measured 8 hours after infection with the indicated adenovirus at an MOI of 10. (D) Ad.SPDD showed improved cancer specificity over ZD55. MRC-5 and Huh-7 cells were infected with Ad.SPDD and ZD55 at the indicated MOIs. Cell viability was measured 4 days post infection. The results are presented as the mean+SD (n = 6). * indicates P < 0.05, *** indicates P < 0.001, and NS means not significant (with a P value > 0.05).

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