Genomic amplification and overexpression of MDR1, and a number of miRNA regulation of TP53INP1. (A) Twenty fold amplification of multi-drug resistance gene (MDR1) region in MCF7-ADR as compared with MCF7. (B and C) Overepression of MDR1 gene in MCF7-ADR by microarray and real-time PCR, respectively. (D) Scatter plot of miRNA target genes predicted by Targetscan. More than 15% of miRNAs up-regulated in MCF7-ADR (74 miRNAs) were predicted to potentially bind the 3'-UTR of these genes. Expression levels of three (TP53INP1, PURB and TRPS1) of them are clearly dwon-regulated. (E) Scatter plot of miRNAs that might bind to 3'UTR of TP53INP1 gene. (F and G) Confirmation of TP53INP1 gene, miR-130 and miR155 expression by real-time PCR. Standard deviation was calculated in triplicate determinants in the experiment.