Pathway enrichment analysis of up- and down-regulated miRNAs in breast cancer cells. (A) Diagram of GO analysis of MCF7 and MCF7-ADR. In the comparison with MCF7 and MCF7-ADR, 5, 137 genes and 3, 579 genes are predicted as miRNA target candidates of 74 up-regulated miRNAs and 32 down-regulated miRNAs, respectively. With these predicted genes, enriched pathways are selected, as shown Table S1. (B) Wnt signaling pathway. 74 miRNA-targeted genes are plotted in Wnt signal pathway map (left), 32 miRNA-targeted genes are plotted (right). Red boxes show high expression genes, and green boxes show low expression genes.