Akt3, whose expression was inversely correlated with miR-505, is associated with drug resistance in MCF7-ADR cells. (A) Scatter plot of 2 probes of Akt3 genes that up-regulated in MCF7-ADR cells. (B) Real-time RT-PCR analysis for Akt3 in miR-505 or control miRNA-transfected cells. Seventy-two hours after transfection with miR-505 or control miRNA, Akt3 expression levels were determined. Expression levels were normalized to GAPDH expression levels. N = 9, mean ± S.D. is shown. (C) Down-regulation of Akt3 induced cell growth arrest in MCF7-ADR cells at 1, 10 and 20 nM DOC conditions. Cell growth was calculated by luciferase activity. n = 3, ∗, P < 0.05. ∗∗, 0.01.