Figure 3
From: Retinoic acid pathway activity in wilms tumors and characterization of biological responses in vitro
Retinoids affect proliferation and cell morphology. (A) Cell cultures ws539, ws568 and ws591 exhibit a decreased growth rate when treated with ATRA, 9cis RA or 4HPR. Cultures shown represent examples since different individual cultures derived from the same tumor sample/patient reacted in the same way. In culture ws592 no clear reduction of proliferation could be observed, except for 4HPR treatment. (B) Morphological changes under RA treatment as observed in WT cell culture ws568li. ATRA and 9cis RA induced a pronounced increase of cell size and formation of actin stress fibers. 4HPR rather led to cell shrinkage and detachment. Actin cytoskeleton is visualized in green (Phalloidin-FITC), nuclei are stained with Hoechst 33342 (blue), scale bar: 50 μm. (C) Numerical representation of the cell size changes upon treatment in cell lines ws539A, ws568li and ws591.