Development of non-apoptotic membrane protrusions in SOX2-silenced U343-MG and U373-MG cells. a: Representative microscopy images showing U343-MG cells with knockdown of SOX2 and control cells transduced with shLuc. b: Quantification of the fraction of cells showing membrane blebs after knockdown of SOX2. Bars indicate standard deviation. c: Confocal laser scan micrsocopy images showing Phalloidin-TRITC staining of actin filaments in U343-MG and U373-MG cells after knockdown of SOX2. CU: Close ups on cortical actin filaments. As control shLuc transduced U343-MG and U373-MG cells are included. Note the increased thickness of cortical actin filaments (arrowheads) and the development of membrane alterations in cells with knockdown of SOX2. d:. Analysis of the thickness of cortical actin filaments: The thickness of the cortical actin network is increased in cells with knockdown of SOX2 when compared to U343-MG and U373-MG shLuc control cells. * p < 0.05; **p > 0.01 when SOX2-silenced cell were compared to shLuc controls.