SOX2-RNAi affects adhesion, invasion and migration of glioma cells. a: Trans-well assay measuring the invasion of U343-MG- and U373-MG glioma cells with stable knockdown of SOX2 and of shLuc control cells using extra cellular matrix (ECM)-coated Boyden chambers. The calculation of the proteolysis-dependent invasion index was performed as described in the material and methods section. Bars indicate standard deviation. * p < 0.01 when cells with knockdown of SOX2 were compared to shLuc controls. b: Western blot analysis of focal adhesion kinase (FAK) and its activated phosphorylated form (FAK-pY397). FAK and FAK-pY397 was analyzed in SOX2 silenced (shSOX2-2378) and in control cells (shLuc) at two different time points 3 days (3 d) and 5 days (5 d) after transduction. Note the decrease in FAK-pY397 in cells with knockdown of SOX2. c: Western blot analysis showing diminished expression of the human enhancer of filamentation protein (HEF1/NEDD9), and of pro-matrix metalloproteinases (pro-MMPs) 1 and 2, in U343-MG and U373-MG glioma cells with knockdown of SOX2.