Figure 6

SOX2-RNAi induces morphological changes in glioma cells. Trans well assay using membranes lacking the ECM for determination of proteolysis-independent amoeboid-like migration of a: U343-MG and b: U373-MG cells with knock down of SOX2. The calculation of the amoeboid-like migration index was performed as described in the material and methods section. Bars indicate standard deviation. * p < 0.05; ** p < 0.01 when cells with knockdown of SOX2 were compared to shLuc controls. c-e: U343-MG cell transduced with shSOX2 #2378 and expressing the marker EGFP were monitored by life cell imaging. c: An adherent cell with normal cell morphology develops protrusions of the membrane which appear as little blebs. d: An intensive blebbing cell develops less frequent, but larger blebs, and acquire a round cell morphology; e: A fully rounded cell contracts the cell body and migrates. Magnification bars: 30 μm.