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Figure 4 | Molecular Cancer

Figure 4

From: Targeting notch pathway enhances rapamycin antitumor activity in pancreas cancers through PTEN phosphorylation

Figure 4

Notch Inhibition Results in Downregulation of Akt Activation Through Rho-Dependent PTEN phosphorylation. (A) Notch inhibition with MRK003 led to a decrease in Akt phosphorylation, but not ERK phosphorylation in K399. While the level of PTEN did not change with MRK003 treatment, expression of pPTEN was enhanced. Cdc42, a down-stream target of PTEN was also reduced. (B) MRK003 induced expression of the PTEN regulator, RhoA, but not Rock1. (C) Transcription of RhoA was induced 1.5× with MRK003, suggesting that the induction of RhoA by Notch inhibition is transcriptionally mediated. (*) denotes statistical significance compared with control. (D) This induction of phospho-PTEN was ameliorated in the presence of Rock1 inhibitor Y27632 at 30 μM (lanes 4 and 5), suggesting that Notch's effect on phosphoPTEN depends on the Rock/Rho pathway.

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